APP／PS1双转基因阿尔茨海默病模型小鼠视网膜神经节细胞丢失的初步观察  

Retinal ganglion cell loss on APP/PS1 transgenic mice with Alzheimer＇s disease

作　　者：卢艳[1] 唐娜[1] 王蓉[2] 

机构地区：[1]首都医科大学宣武医院眼科,100053 [2]首都医科大学宣武医院中心实验室,100053

出　　处：《中华眼科杂志》2012年第9期836-841,共6页Chinese Journal of Ophthalmology

基　　金：国家“十一五”863计划“疫苗与抗体工程”子课（2006AA02A245）;北京市卫生系统高层次卫生技术人才培养计划（2009-3-64）

摘　　要：目的研究APP／PS1双转基因阿尔茨海默病（AD）模型小鼠视网膜神经节细胞数目的变化。方法实验研究。选取11个月龄APP／PS1双转基因AD模型雌性小鼠10只作为模型组，同月龄同背景非转基因雌性小鼠10只作为正常对照组。两组小鼠经双侧上丘注射30％辣根过氧化物酶（HRP）逆行标记视网膜神经节细胞。小鼠存活48h后灌注处死，取小鼠大脑做冰冻切片，刚果红染色。取小鼠右眼视网膜并铺片，行视网膜HRP组织化学反应，计算机图像分析系统对视网膜神经节细胞进行计数。取小鼠左眼球做石蜡切片，HE染色，行视网膜各层神经细胞计数。对照组和模型组各层细胞数目的比较采用独立样本t检验。结果模型组小鼠大脑刚果红染色阳性，对照组小鼠大脑刚果红染色阴性。视网膜铺片检测结果显示，对照组与模型组视网膜神经节细胞计数分别为（112．78±7．70）和（78．72±11．35）个／视野，模型组视网膜神经节细胞明显减少（t=27．28，P〈0．01）。石蜡切片检测结果显示，与对照组相比，模型组视网膜内核层与外核层细胞数无明显减少（P〉0．05）；对照组与模型组视网膜神经节细胞层细胞数分别为（8．17±1．17）和（5．17±0．75）个／视野，模型组视网膜神经节细胞层细胞数量较正常小鼠明显减少（t=52．88，P〈0．01）。结论11个月龄APP／PS1双转基因AD模型小鼠视网膜神经节细胞数目减少。Objective To investigate the retinal nerve cell changes in number by tests on APP/PS1 double transgenic mice. Methods Experimental study. Comparison was made between 10 female 11-month- old APP/PS1 double transgenic mice with Alzheimer＇s disease（ model group） and 10 non-transgenic female mice of the same background and age （contral group）. Retinal ganglion cell （RGC） was retrograde labeled by injecting horse radish peroxidase（HRP） into the bilateral optic nerve layer of the superior colliculus of two groups. After 48 hours, all mice were perfused sacrificed. The brains were frozen, mode slices, stained with methyl-alcohol-Congo. The retina peeled from right eye was conducted HRP histochemistry reaction to count RGC by computerized image analyzer. The left eyes were treated with paraffin-embedded, mode slices, stained with hematoxylin and eosin （ HE ） to count the neuron numbers of each retina layers. The neuron numbers of each retina layer for control group and model group were statistically analyzed using t test for independent samples. Results The brains showed positive in the model group and the brains showed negative in the control group after stained with methyl-alcohol-Congo. By counting retinal ganglion cells traced with HRP, the number of RGC was statistical different between two groups at 112. 78 ± 7.70 within per field for the control group against 78.72 ± 11.35 within per field for the model group. The RGC of the model group was comparatively much less than the control group （ t = 27.28, P 〈 0. 01 ）. By counting cells of retina stained with HE, the number of inner and outer nuclear layer cells were not statistical different between two groups of each layer（ P 〉 0. 05 ） , the number of RGC in the control group and the model group were respectively 8.17 ±1.17 within per field,5.17± 0. 75 within per field. The ceils in retinal ganglion layer in the model group were significantly reduced （ t = 52. 88, P 〈 0. 01 ）. Conclusion The results imply that the number of

关 键 词：小鼠 转基因 阿尔茨海默病 辣根过氧化物酶 视网膜神经节细胞 淀粉样Β蛋白前体 

分 类 号：R749.16[医药卫生—神经病学与精神病学]