胃复春对肿瘤坏死因子诱导人胃上皮GES-1细胞炎症和肠化的影响  

Effects of Weifuchun on TNF-α-induced inflammation and intestinal metaplasia in GES-1 cells

作　　者：赵敏[1] 吕宾[1] 黄宣[1] 陈冰冰[1] 金海峰[1] 

机构地区：[1]浙江中医药大学附属第一医院消化科,浙江杭州310006

出　　处：《中国中西医结合消化杂志》2012年第9期394-397,共4页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：浙江省中医药管理局基金资助课题(No:2010ZB045)

摘　　要：[目的]研究胃复春散剂对肿瘤坏死因子α(TNF-α)诱导人胃上皮GES-1细胞炎症及肠化的影响,进而探讨胃复春治疗慢性胃炎的可能机制。[方法]体外培养人胃上皮细胞GES-1,用ELISA法检测白细胞介素(IL)-6、IL-4的表达,用RT-PCR法检测细胞表面肠黏膜标志物黏蛋白2(MUC2)及胃黏膜标志物性别决定区Y框蛋白2(SOX2)mRNA的表达。[结果]胃复春可上调细胞表面IL-4的表达,抑制IL-6的表达,下调细胞中MUC2mRNA的表达,上调SOX2mRNA的表达,且药物治疗组与模型组相比均P<0.05。[结论]胃复春可能通过上调IL-4的表达及抑制IL-6的表达来实现抑制TNF-α诱导GES-1细胞炎症;通过下调MUC2和上调SOX2的表达来实现抑制肠化的过程,这为中药胃复春对慢性胃炎的防治作用提供了新的证据。[Objective]To explore the effects of Weifuchun on TNF-α-induced inflammatory and intestinal metaplasia of GES-1 cells and to discuss the possible mechanism of the treatment of chronic gastritis.[Methods] Human gastric epithelial GES-1 cells were cultivated.ELISA was applied to test the levels of IL-4 and IL-6 and RT-PCR to test the expression of MUC2 and SOX2 mRNA.[Results]Weifuchun increased the level of IL-4 and decreased that of IL-6 while it decreased the expression of MUC2 mRNA and increased that of SOX2 mRNA.The difference between the drug treatment group and model group was significant（P〈0.05）.[Conclusion]Weifuchun could inhibit the process of inflammatory in GES-1 cells by increasing the level of IL-4 and decreasing that of IL-6.It could suppress the process of intestinal metaplasia by decreasing MUC2 expression and increasing SOX2 expression.The study provided new evidence for Weifuchun in preventing and treating chronic gastritis

关 键 词：胃炎 慢性 胃复春 肿瘤坏死因子 白细胞介素6 白细胞介素4 

分 类 号：R573.3[医药卫生—消化系统]