缺氧时三氧化二砷对5-氟尿嘧啶抗肝癌细胞株BEL-7402敏感性的影响  

Effect of 5-fluorouracil (5-FU) Joint Arsenic Trioxide (As_2O_3) on Growth Inhibition of Liver Cancer Cell BEL-7402 During Hypoxia in Vitro

作　　者：刘铁夫[1] 刘晓川 刘勇[1] 赵丽莉[1] 崔宝红[3] 周婷[1] 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001 [2]北京市煤炭总医院,北京100028 [3]哈尔滨医科大学附属第三医院,黑龙江哈尔滨150040

出　　处：《黑龙江医学》2012年第9期641-642,共2页Heilongjiang Medical Journal

基　　金：黑龙江省自然科学基金项目(A)D2007-96;黑龙江省卫生厅科研项目2006-236

摘　　要：目的探讨缺氧条件下,不同浓度的5-氟尿嘧啶(5-FU)联合0.5μmol/L浓度三氧化二砷(As2O3)对肝癌细胞株BEL-7402生长抑制的影响。方法用二氯化钴(CoCl2)诱导细胞培养的化学性缺氧条件,及四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法测定缺氧条件下不同浓度5-FU及5-FU联合As2O3对肝癌细胞BEL-7402的生长抑制作用。结果在化学性缺氧条件下,不同浓度5-FU联合As2O3对肝癌细胞株BEL-7402生长抑制作用比单用5-FU有统计学差别(P﹤0.05),呈剂量-效应依赖关系。结论在CoCl2诱导的化学缺氧条件下,非细胞毒剂量As2O3具有改善缺氧状态下5-FU对人肝癌细胞BEL-7402化疗敏感性下降的作用。Objective To study Effect of 5 - fluorouracil （5 - FU） ） joint 0. 5 umol/L concentrations of arsenic trioxide （As2O3 ） on growth inhibition of liver cancer cell BEL -7402 during hypoxia the. Methods The envi- ronment of hypoxia was induced by CoCl2 The effect of 5 -FU joint As2O3, 5 -FU determined by MT. Results In chemistry under hypoxic conditions, 5 - FU in different concentration combined with As2O3 on hepatoeellular carcinoma growth inhibition of BEL - 7402 than 5 - FU alone there was statistically significant difference （ P 〈 0.05 ）, dose- effect dependencies. Conclusion In chemical hypoxia induced by CoCl2 non -cytotoxie dose of As2O3 could improved 5 - FU declining role on inhibit growth in BEL -7402 human liver cancer cells.

关 键 词：三氧化二砷 5-氟尿嘧啶 缺氧 MTT法 

分 类 号：R730.53[医药卫生—肿瘤]