气相色谱-质谱联用法检测中毒酒样中的钩吻碱  

Determination of Gelsemine in Poisoning of Liquor Samples by Gas Chromatography Mass Spectrometry

作　　者：张瑞[1,2] 雷宁生[1] 林莹[2] 苏小川[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028 [2]广西大学轻工与食品工程学院,广西南宁530004

出　　处：《广西科学》2012年第3期239-240,243,共3页Guangxi Sciences

摘　　要：用气相色谱-质谱联用法(GC-MS)测定引起中毒的定性含有钩吻素的自酿药酒样品。样品用自制无水硫酸钠小柱干燥净化后,在DB-5MS(30m×0.25mm×0.25μm)毛细管色谱柱上分离,用气-质联用仪进行测定。结果样品定性检出钩吻素甲,色谱分离效果良好。本方法简单、快捷,灵敏度高,测定结果准确、可靠,适合日常钩吻碱中毒样品的检测。To separate and determine gelsemine from the wine in a poisoning case by means of GC/MS. After being dried over anhydrous sodium sulfate, purified samples were separated on a DB-SMS column（30m× 0. 25mm ×0. 25μm） and detected by gas chromatography with mass spectrometry. The denied gelsemine was found in the case sample, and the chromatographic separation of the peaks is fine. The method is simple, fast, and sensitive with accurate and reliable results,which is suitable to conduct routine determination of gelsemine from poisoning incident.

关 键 词：钩吻碱 中毒 气相色谱-质谱联用 

分 类 号：O657.63[理学—分析化学]