PAI-1在TGFβ_1基因和反义TGFβ_1基因转染人肝癌细胞株中的表达  

Expression of PAI-1 on human hepatoma cells transfected by TGF-β_1 gene and antisense-TGF-β_1 vector

作　　者：林文生[1] 张农[1] 张颂文[2] 顾建新[2] 郭慕依[1] 

机构地区：[1]上海医科大学病理学教研室,200032 [2]上海医科大学基因研究中心,200032

出　　处：《临床与实验病理学杂志》2000年第3期221-223,共3页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家"九五"攻关项目!(96 90 6 0 1 1 5);上海市教委重点学科基金;CMB基金!(93 583)资助项目

摘　　要：目的 :通过对人肝癌细胞株 (SMMC 772 1)转染TGF β1基因及反义TGF β1基因 ,观察该细胞PAI 1表达的变化。方法 :采用电穿孔法进行基因转染 ,并用Westernblot、Northernblot法加以鉴定后 ,分别应用Westernblot及Northernblot观察该细胞表达PAI 1mRNA的变化。结果 :过表达的TGF β1细胞克隆PAI 1mRNA表达均高于对照组 ,而低表达TGF β1者PAI 1mRNA表达低于对照组。结论 :TGF β1可能通过上调肝癌细胞PAI 1的表达 ,在肝癌浸润和转移过程中发挥重要作用。Purpose To study the expression of PAI 1 on human hepatoma cells (SMMC7721) transfected by TGF β 1 gene and antisense TGF β 1 vector. Methods Electroporation for gene transfection, which was confirmed by Western blot and Northern blot; Northern blot and Western blot analysis were used for detecting the expression of PAI 1 on transfectants. Results Higher and lower mRNA levels of PAI 1 were observed in SMMC 7721 cells transfected with TGF β 1 gene and antisense TGF β 1 vector, respectively. Conclusion It is possible that TGF β 1 is involved in the invasion and metastasis of human hepatoma by upregulating PAI 1 expression.

关 键 词：肝肿瘤 TGF-β1 PAI-1 基因转染 肿瘤侵润 

分 类 号：R735.702[医药卫生—肿瘤] R73-37[医药卫生—临床医学]