利用T细胞受体变异β基因谱系分析T细胞急性淋巴细胞白血病病人T细胞克隆性(英文)  

Analysis of T cell clonality by using T-cell receptor varible β gene repertoire in T-cell acute lymphoblastic leukemia

作　　者：李杨秋[1] 陈少华[1] 杨力建[1] 祁明芳[1] 

机构地区：[1]广州暨南大学医学院血液病研究室,广东广州510632

出　　处：《中国病理生理杂志》2000年第7期627-632,共6页Chinese Journal of Pathophysiology

基　　金：thegrantsfromthenationalnaturalsciencefoundationofChina! (No .39870 35 8) ;thenaturalsciencefoundationofGuangdongprovince!

摘　　要：目的 :分析T细胞 -急性淋巴细胞白血病 (T -ALL)病人的T细胞克隆性。方法 :利用RT -PCR方法分析 6例T -ALL和 10例正常人外周血单个核细胞中 2 4个T细胞受体变异 β(TCRVβ)基因的CDR3长度 ,PCR产物再进一步进行基因扫描和序列分析。结果 :3例病人的某些TCRVβ亚家族T细胞呈单克隆或寡克隆性增殖 ,主要为Vβ2、3、6、9、2 1和 2 4。其它 3例及正常人均表现为多克隆性增殖T细胞。 结论 :部分T -ALL来自于TCRVβ亚家族克隆性增殖T细胞。AIM: To analyze T cell clonality in patients with T cell acute lymphoblastic leukemia (T-ALL). METHODS: The complementarity determining region 3 (CDR3) size of 24 T cell antigen receptor variable β (TCR Vβ) region gene was analyzed in peripheral blood mononuclear cell (PBMC) samples from 6 T-ALL cases and 10 normal individuals by using reverse transcriptase-polymerase chain reaction (RT-PCR). The PCR products were further studied by genescan and sequencing analysis. RESULTS: Some TCR Vβ subfamily T cells display mono- or oligoclonal expansions in 3 cases of T-ALL, predominantly in Vβ2, Vβ3, Vβ6, Vβ9, Vβ21 or Vβ24, respectively. Polyclonal expansions of T cells were found in the other three cases, which could also be found in normal samples. CONCLUSION: A part of T cell acute lymphoblastic leukemia cells may arise from a clonal expansion of TCR Vβ subfamily T cell. This method may be useful for the detection of minimal residual disease in clinical study of the disease.

关 键 词：T细胞-急性淋巴细胞白血病 T细胞克隆性 TCR-Vβ 

分 类 号：R733.710.2[医药卫生—肿瘤]