万古霉素手性选择剂结合区段灌注技术的氨基酸毛细管电泳手性分离  

Capillary Electrophoretic Chiral Separation of Amino Acids Based on the Combined Use of Vancomycin as Chiral Selector and Segmented Filling Technique

作　　者：申刚义[1] 佘东来[1] 张爱芹[2] 

机构地区：[1]中央民族大学中国少数民族传统医学国家民委-教育部重点实验室,北京100081 [2]中央民族大学生命与环境科学学院,北京100081

出　　处：《理化检验（化学分册）》2012年第9期1008-1011,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金(81001595);教育部"长江学者和创新团队发展计划"(IRT0871);中央高校基本科研业务费专项资金中央民族大学自主科研项目(0910KYQN67)

摘　　要：结合区段灌注技术,以万古霉素为手性选择剂构建毛细管电泳手性分离体系,研究了氨基酸对映异构体的分离。选择的最佳分离条件为:①含3mmol.L-1万古霉素和2mmol.L-1HDB的pH 7为200mmol.L-1硼酸缓冲溶液的分离区段;②灌注长度为压力3.45kPa,时间2.5min。以含2mmol.L-1 HDB的pH 7为200mmol.L-1硼酸缓冲溶液为运行缓冲溶液对19种氨基酸衍生物进行手性分离。试验结果显示其中15对氨基酸对映异构体得到了良好分离。Separation of enantiomers of amino acids was studied by the capillary electrophoretic chiral separation system established on the base of combined use of segmented filling technique and vancomycin as ehiral selector. The optimum separation conditions were selected as follows： （1） a solution of 200 mmol·L-1 H3BO3 buffer of pH 7 containing 3 mmol·L-1 vancomycin and 2 mmol ·L-1 HDB was placed in the separation segmented section with the H3BO3 solution of HDB as conveying solution; （2） the length of segmented filling was kept under a pressure of 3. 45 kPa through a time of 2. 5 min. As shown by the experimental results, 15 pairs of the enantiometric amino acids among the 19 derivatives of amino acids tested were well separated.

关 键 词：毛细管电泳 手性分离 氨基酸 万古霉素 区段灌注技术 

分 类 号：O657.8[理学—分析化学]