山东地区肾综合征出血热患者基因诊断分型研究  

Study of Genomic Typing for HFRS in Patients in Shandong District

作　　者：辛崇尚[1] 马立宪[1] 邵丽华[1] 王刚[1] 吴世英[1] 

机构地区：[1]济南市传染病医院,现为山东医科大学硕士研究生250021

出　　处：《山东医药》2000年第12期1-3,共3页Shandong Medical Journal

基　　金：山东省卫生厅立项课题! (No.96 85 )

摘　　要：采用逆转录聚合酶链反应 ( RT- PCR) ,对山东地区 60例肾综合征出血热 ( HFRS)患者的临床标本进行扩增 ,采用酶切分析鉴定扩增产物的特异性。结果 :60例标本均呈 型或 (和 ) 型阳性 ,扩增片段的大小与理论值一致。表明山东地区流行的 HFRS 型和 型毒株同时存在 ,以 型为主 ;RT-PCR是一项较特异。To identify the genomic types of Hantaviruses,4 different sets of type specific primers based on the unique G 1 sequences M genomic segments of HTNV and SEOV were designed Total cellular RNA extracted from clinical sample of HFRS patients in Shandong district was RT to cDNA and amplified by PCR assay The specification of products were then verified by restriction enzyme analysis The results showed that all samples were amplified by type Ⅰ primer or/and type Ⅱ primer The results were consistent with those of serological typing as reported It suggested that RT PCR is a sensitive,specific method for typing of Hantaviruses

关 键 词：肾综合征出血热 RT-PCR 发型分型 基因诊断 

分 类 号：R512.804[医药卫生—内科学]