紫外诱变选育β-呋喃果糖苷酶高产菌株及培养基优化  

UV mutagenesis for breeding high-yield strains of β-fructofuranosidase and optimization of culture medium

作　　者：马歌丽[1] 韩甜甜[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002

出　　处：《饲料工业》2012年第18期53-58,共6页Feed Industry

基　　金：河南省教育厅科技攻关项目[2011A530011];郑州轻工业学院重大预研基金项目[xyyjj02]资助

摘　　要：以黑曲霉为出发菌株,通过紫外诱变,筛选得到一株产β-呋喃果糖苷酶活力较高的突变株YA03,其产酶活力是出发菌株的1.66倍,且遗传稳定性良好。采用单因素和响应面法对该突变株产β-呋喃果糖苷酶的发酵培养基进行优化,得到最佳产酶培养基:蔗糖11.86%、牛肉膏0.53%、MnCl2.4H2O 0.14%、MgSO.47H2O 0.05%、FeSO.47H2O 0.05%,此条件下β-呋喃果糖苷酶活力可达96.58 U/ml。Aspergillus niger for the original strain,the mutation strain YA03 producing high β-fructofuranosidase activity was screened by UV mutagenesis,its enzyme activity is 1.66 times than the original strain with genetic stability.The mutagenesis strains producing β-fructofuranosidase of fermentation medium used univariate and response surface method to optimize the best medium：sucrose 11.86%,beef extract 0.53%,MnCl2·4H2O 0.14%,MgSO4·7H2O 0.05%,FeSO4·7H2O 0.05%.Under these conditions,β-fructofuranosidase activity reached 96.58 U/ml.

关 键 词：Β-呋喃果糖苷酶 黑曲霉 紫外诱变 响应面法 培养基优化 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]