气相色谱-四极杆飞行时间串联质谱在加拿大一枝黄花挥发性成分检测中的应用  

Application of GC-QTOF MS in Identification of Volatile Components in Solidago Canadensis L.

作　　者：范若静[1] 张芳[1] 郭寅龙[1] 

机构地区：[1]中国科学院上海有机化学研究所上海质谱中心,上海200032

出　　处：《分析测试学报》2012年第9期1051-1057,共7页Journal of Instrumental Analysis

基　　金：创新方法工作专项项目(2011IM030700);国家自然科学基金项目(21072215)

摘　　要：采用气相色谱-四极杆飞行时间串联质谱(GC-QTOF MS)对加拿大一枝黄花中的挥发性成分进行检测。利用气相色谱的保留指数、四极杆飞行时间串联质谱的高分辨多级质谱数据以及谱库检索等对该植物的叶、茎、根三个部位中的挥发性成分进行结构鉴定。共鉴别出挥发性成分54种,其中叶47种、茎35种、根32种。主要成分均为蒎烯、表双环倍半水芹烯、柠檬烯等萜烯类化合物。结果表明,气相色谱的高分离度和四极杆飞行时间串联质谱的高分辨率可以有效地对复杂样品中的挥发性成分进行检测,结合多维数据定性技术能够显著提高未知成分鉴别的准确性。The volatile components in the leaf,stem and root of Solidago Canadensis L.were analyzed by gas chromatography-quadrupole time of flight mass spectrometry（GC-QTOF MS）.Total of fifty four compounds were found using a multidimensional qualitative analysis approach consisting of NIST MS search,accurate mass measurement retention index and area normalization.Terpenes such as limonene,pinene,germacrene D were identified as the major volatile components existing in the root,stem and leaf of Solidago Canadensis L.Results showed that GC-QTOF MS with high separation efficiency and high mass accuracy could be widely applied in the detection of the volatile components in complicated matrices.And the multidimensional qualitative approach could simplify molecular characterization and structural confirmation.

关 键 词：气相色谱-四极杆飞行时间串联质谱(GC-QTOFMS) 结构鉴别 加拿大一枝黄花 挥发性成分 

分 类 号：O657.63[理学—分析化学] TQ460.72[理学—化学]