胶束电动毛细管色谱同时测定食品中13种人工合成色素  

Determination of Thirteen Artificially Synthesized Pigments in Food by Micellar Electrokinetic Capillary Chromatography

作　　者：龙巍然[1,2] 王兴益[2] 史振雨[2] 岑怡红[2] 白玉[1] 刘虎威[1] 

机构地区：[1]北京大学化学与分子工程学院,北京100871 [2]兴义民族师范学院化学与生物系,贵州兴义562400

出　　处：《分析测试学报》2012年第9期1100-1104,共5页Journal of Instrumental Analysis

基　　金：北京市科技项目(Z111100056811006)

摘　　要：建立了胶束电动毛细管色谱(MEKC)同时测定食品中10种水溶性和3种脂溶性人工合成色素的分析方法。应用正交法优化了缓冲溶液的pH值、硼酸浓度和SDS加入量,并研究了有机改性剂、分析电压、毛细管温度等对分离的影响。确定最佳电泳条件为:未涂层弹性石英毛细管柱(75μm×58.5 cm),缓冲溶液为40 mmol/L硼酸-氢氧化钠(pH 9.5),加入20 mmol/L的SDS和30%的乙腈作改性剂,检测波长为220 nm,分析电压25 kV,毛细管温度25℃,进样压力50 MPa,进样时间5 s。在优化条件下,13种合成色素在3～200 mg/L范围内线性关系良好,r2均大于0.99。将该方法用于分析市售卤翅、腐竹、番茄酱、辣椒面等样品,其回收率为98%～104%,检出限为3.0～16.0 mg/L。该方法简便、准确,能够满足食品中合成色素的常规检测要求。A micellar electrokinetic capillary chromatographic（MEKC） method was developed for the separation and determination of 13 artificially synthesized pigments,including ten water-soluble and three fat-soluble substances（e.g.auramine O,Sudan Ⅱ,Sudan Ⅰ,Sudan ⅢⅣ,acid orange,brilliant,acid red 92,erythrosine,uranine,allura red,acid red 1,indigo and sunset yellow） in food.The parameters including buffer composition,pH value,concentration of boric acid and SDS were optimized by orthogonal experiment method.At the same time,effects of organic modifier,separation voltage and temperature on the separation were investigated.The optimal conditions were as follows：separation column：a 75 μm×58.5 cm uncoated fused silica capillary;buffer：40 mmol/L sodium borate（pH 9.5）;BGE solution：20 mmol/L SDS-30% acetonitrile;detection wavelength：220 nm,run voltage：25 kV;temperature：25 ℃,and the sample was injected at 50 MPa for 5 s.Under the optimal conditions,the linear relationship between concentration and peak area for each of these analytes was obtained in the concentration range of 3-200 mg/L,with correlation coefficients larger than 0.99.The method was applied in the determination of 13 artificially synthesized pigments in samples of spiced duck wings,dried bean milk cream in tight rolls,ketchup and chili powder,with recoveries between 98% and 104%.The limits of detection ranged from 3.0 mg/L to 16.0 mg/L.The results indicated that the method was simple and accurate,and was suitable for the simultaneous determination of the thirteen pigments in food samples.

关 键 词：胶束电动毛细管色谱法(MEKC) 人工合成色素 食品 

分 类 号：O657.8[理学—分析化学] TQ047.2[理学—化学]