微波及水浴对两种临时冠材料细胞毒性的影响  

Effect of microwave and water-bath post-polymerization treatments on cytotoxicity of two kinds of provisional restoration materials

作　　者：龚丽琴[1] 张怀勤[1] 马骏驰[2] 高传飞[1] 

机构地区：[1]南京医科大学口腔医学研究所.附属口腔医院修复科,江苏南京210029 [2]南京医科大学附属口腔医院特诊科,江苏南京210029

出　　处：《口腔生物医学》2012年第3期139-141,共3页Oral Biomedicine

基　　金：江苏省领军人才与创新团队基金(ZCB2012019);江苏高校优势学科建设工程资助项目

摘　　要：目的:探讨微波和水浴处理对聚甲基丙烯酸甲酯和双甲基丙烯酸复合树脂临时冠材料细胞毒性的影响。方法:两种材料分别制备直径10mm厚2mm圆柱体18个,每种材料分3组,每组6个试件,分别为微波组、水浴组、对照组。对照组不进行处理;微波组在500W微波条件下处理3min;水浴组在55°C水中恒温浸泡30min。获取试件浸提液后采用甲基噻唑基四唑(MTT)法检测细胞毒性。结果:聚甲基丙烯酸甲酯细胞相对增殖率:微波组(78.4%±4.3%)和水浴组(54.4%±4.3%)均大于对照组(47.6%±3.6%),结果有统计学差异,各组间两两比较也有统计学差异(P<0.05)。双甲基丙烯酸复合树脂材料细胞相对增殖率:三组间比较无统计学差异(P>0.05),细胞毒性分级皆为1级。结论:微波和水浴处理可有效降低聚甲基丙烯酸甲酯临时冠材料细胞毒性,对双甲基丙烯酸复合树脂临时冠材料无明显作用。Objective：To evaluate the effect of microwave and water-bath post-polymerization treatments on the cytotoxicity of two kinds of provisional restoration materials, polymethyl methacrylate and bis-acryl composite resin. Methods：18 cylinders （10 mm in di- ameter and 2 mm in thickness） for assessing cytotoxicity were prepared in each material and divided into three groups, with 6 specimens in each. The three groups were mierowava group, water-bath group, and control group. Specimens in the control group were left untreated. Specimens in microwave group were treated with microwave irradiation for 3 min at 500 W. In water-bath group, specimens were submitted to immersion in water at 55~C for 30 min. Leaching liquor of the specimens was used to assess the cytotoxicity of cells by means of methyltetrazolium test. Results： For polymethyl methacrylate material, the mean percentage viability of control group （47. 6 ± 3.6） % was signifi cantly lower than those of microwave group （78.4 ± 4.3 ） % and water -bath group （54.4± 4.3 ） %. There was significant difference between each pair of the three groups （ P 〈 0.05 ）. For bis-acryl composite resin, there was no statistcally difference in the mean percentage of viability among the three groups （ P 〉 0.05 ）, and cytotoxic was in grade I. Conclusions： Microwave and water-bath post-polymerization treatments can reduce the cytotoxicity of polymethyl methacrylate material significantly, while have little effect on the bis-acryl composite resin.

关 键 词：临时冠 微波 水浴 细胞毒性 

分 类 号：R783.1[医药卫生—口腔医学]