SD大鼠下颌下腺主导管结扎损伤及再通后的组织学观察  

Histologic observation of submandibular gland after duct-ligation-damaged and duct reopened in SD rats

作　　者：潘光华[1] 陈伟[2] 张霓霓[2] 王军胜[2] 黄桂林[2] 

机构地区：[1]安顺市人民医院,561000 [2]遵义医学院附属口腔医院

出　　处：《实用口腔医学杂志》2012年第5期570-573,共4页Journal of Practical Stomatology

基　　金：贵州省优秀人才省长基金资助[编号:(2008)113];贵州省教育厅重点项目基金(编号:[2009]0112);2010年度遵义医学院硕士启动基金(编号:F-405)

摘　　要：目的:建立主导管结扎的SD大鼠下颌下腺组织损伤模型,观察主导管结扎及再通后腺体的组织学变化。方法:双重结扎SD大鼠下颌下腺主导管。部分腺体在6 d后摘取;其他腺体于6 d后松开结扎使再通,并于4、6、12、24 d后处死动物,取出腺体。通过HE及免疫组织化学染色观察其组织化学变化。结果:主导管结扎6 d后,腺体中腺泡消失,导管样结构增生,导管细胞表达CK19;在增生导管及周围见α6β1、LN阳性细胞。松开结扎再通的导管在6 d后有成熟的腺泡细胞出现,24 d后有大量成熟腺泡形成,Amylases、PAS(periodic acid-schiff)、AQP-5均在腺泡细胞中表达。结论:导管结扎损伤后腺体腺泡萎缩,导管上皮增生。松开结扎再通后腺泡细胞再生、功能恢复。Objective： To observe the histologic changes when the main duct of submandibular gland was ligated and released in SD rats. Methods： The main duct of submandibular gland was reopened after double ligation for 6 days. Some ligated submandibular glands were harvested at day 6 after ligation. Others were reopened and harvested 4, 6, 12 and 24 days after releasing respectively. The histological changes were observed by HE staining and immunohistological staining. Results： After the main duct ligation, acinar cells disappeared with ductal cell proliferation that expressed CK19. The α6β1, LN positive cells were found in proliferative duct and periductal areas. Acinar cells was appearing at day 6 after release of the ligation. 24 days after releasing, large number of acinar cells expressing amylases, PAS and AQP-5 were formed. Conclusion： After duct ligation, acinar ceils disappear and duct epithelium cells proliferate. After release of the ligation, the acinar ceils regenerate and the function of the gland recovers.

关 键 词：SD大鼠下颌下腺 导管结扎 再通 损伤 组织学 

分 类 号：R780.2[医药卫生—口腔医学]