Er,Cr:YSGG激光备洞对兔牙髓组织热休克蛋白70表达的影响  

Expression of HSP70 in rabbit dental pulp after cavity preparation with Er,Cr:YSGG laser

作　　者：杨超[1] 贾兴亚[1] 安欣[1] 王抒智[1] 钟鸣[1] 

机构地区：[1]中国医科大学口腔医学院,沈阳110002

出　　处：《现代口腔医学杂志》2012年第5期330-334,共5页Journal of Modern Stomatology

基　　金：辽宁省医学高峰建设工程重点科研项目(200909)

摘　　要：目的观察Er,Cr:YSGG激光用于牙体窝洞制备后兔牙髓的形态学变化及热休克蛋白70(heat shockprotein70 HSP70)的表达情况。方法用Er,Cr:YSGG激光在家兔前牙制备V类洞并常规充填,普通高速牙钻备洞作对照,分别于术后4h、24h处死实验动物,组织切片经HE染色及免疫组化染色,镜下观察。结果 Er,Cr:YSGG激光备洞后牙髓轻到中度炎症反应,与牙钻对照组无明显差异。备洞后24h,激光组成牙本质细胞HSP70中到强阳性表达,成纤维细胞、血管内皮细胞中度阳性表达。牙钻组成牙本质细胞及相邻成纤维细胞强阳性表达,血管内皮细胞强阳性表达,对照组HSP70表达高于Er,Cr:YSGG激光实验组,差别有统计学意义(P<0.05)。结论 Er,Cr:YSGG激光备洞对牙髓刺激小,有很好的临床使用价值。Objective To observe the dental pulpal morphological changes and the expression of HSP70 to cavity preparation in rabbit incisors by the Er,Cr：YSGG laser.Methods A total of thirtyt-wo incisors of eight healthy rabbits were divided into two groups：Er,Cr：YSGG laser and conventional bur group.after the cavities preparation by the laser and the conventional bur in rabbit incisors,the cavities were filled by normal way.The teeth were extracted 4 and 24 hours after operation respectively,after HE and immunohistochemical staining,the tissue sections were observed by microscopy.Results The histological examination showed that there was no appreciable difference in the manner in which dental pulp tissue responds to cavity preparation with either Er,Cr：YSGG laser or a conventional bur.24h after the operation,the Er,Cr：YSGG laser group have a moderate to strong expression of HSP70 in Odontoblasts,and moderate expression in Fibroblast and endothelial cells.the expression of HSP70 in the control group was higher than that in the Er,Cr：YSGG laser group（P 0.05）.Conclusion Cavity preparation by Er,Cr：YSGG laser causes no noticeable pulpal damage to the dental pulp tissue,and have great clinical value.

关 键 词：Er Cr:YSGG激光 热休克蛋白70 牙髓 备洞 

分 类 号：R781.05[医药卫生—口腔医学]