难治性颞叶癫痫海马星形胶质细胞中P-gp、p53表达与凋亡探讨  

Expression of P-gp, p53 and apoptosis in the hippocampal astrocyte in patients with refractory temporal lobe epilepsy

作　　者：段宝奇[1] 刘忆[2] 胡静[1] 高觉民[1] 

机构地区：[1]江苏省中医院神经外科,江苏南京210029 [2]江苏省中医院血液净化中心,江苏南京210029

出　　处：《海南医学》2012年第19期1-3,共3页Hainan Medical Journal

摘　　要：目的探讨难治性颞叶癫痫海马星形胶质细胞中P-gp、p53的表达和凋亡的意义。方法对17例难治性颞叶癫痫患者(实验组)和3例严重颅脑外伤、2例半球脑梗塞患者(对照组)的手术标本分别进行免疫组化检查P-gp、p53;原位末端标记检测细胞凋亡;免疫印迹法检测P-gp、p53。结果对照组P-gp主要在血管内皮细胞表达,未见胶质细胞有p53和凋亡表达;实验组P-gp分布在血管内皮细胞和星形胶质细胞中,14例有p53和凋亡在星形胶质细胞表达;免疫印迹法P-gp,p53表达较对照组明显增加。结论难治性颞叶癫痫海马星形胶质细胞中有P-gp、凋亡异常表达,p53可能发挥了调节作用。Objective To explore the expression of P-gp, p53 and apoptosis in the hippocampal astrocyte in patients with refractory temporal lobe epilepsy. Methods The specimens were obtained from 17 patients with refrac- tory temporal lobe epilepsy. The expression of P-gp, p53 was measured by immunohistochemistry and Western blot. The level of apoptosis was determined by TdT-mediated dUTP Nick-End Labeling. Results P-gp and p53 were high- ly expressed in the hippocampal astrocyte. Increased apoptosis level of hippocampal astrocyte was also observed. Conclusion The expression of P-gp, p53 and the level of apoptosis is significantly increased in the hippocampal as- trocyte. P53 may play a role during this process.

关 键 词：癫痫 星形胶质细胞 P糖蛋白 凋亡 p53 

分 类 号：R742.1[医药卫生—神经病学与精神病学]