南极微生物絮凝剂产生菌的筛选、分子鉴定及适应性研究  

Screening,Molecular Identification and Adaptability Exploration of Antarctic Microbial Flocculant-Producing Bacteria

作　　者：杜宁[1] 

机构地区：[1]国家海洋局第一海洋研究所 海洋活性物质重点实验室,山东青岛266061

出　　处：《化学与生物工程》2012年第9期40-42,共3页Chemistry & Bioengineering

基　　金：国家海洋局第一海洋研究所基本科研业务费专项资金项目(2010G15)

摘　　要：从南极海水、海冰、底泥中分离得到7株产絮凝剂的菌株,通过16SrRNA基因序列扩增方法进行分子鉴定,分别隶属于不动杆菌属(Acinetobacter)、节杆菌属(Arthrobacter)、嗜冷杆菌属(Psychrobacter)、沙雷菌属(Serratia)、假单胞菌属(Pseudomonas)和假交替单胞菌属(Pseudoalteromonas)。改变培养基中碳源和氮源的组成并观察絮凝率的变化,筛选出在经济培养基条件下具有较高絮凝活性、同时对碳源和氮源具有普适性的菌株I-2和P2-13-1。Seven microbial flocculant-producing bacteria were separated from antarctic seawater, sea ice and marine sediment. The molecular identification of these bacteria was by the way Of 16S rRNA gene sequence amplification. Phylogenetic analysis showed that they belonged to Acinetobacter,Arthrobacter, Psychrobacter, Serratia,Pseudomonas and Pseudoalteromonas, respectively. Different carbon sources and nitrogen sources were used to culture the bacteria in order to observe the change of flocculation activity. The bacteria, I-2 and P2-13-1, were of universal adaptability to different carbon sources and nitrogen sources and had higher flocculation activity in economic culture medium.

关 键 词：微生物絮凝剂 16S RRNA 絮凝率 培养基 

分 类 号：Q939.3[生物学—微生物学]