Determination of Cholesterol Content in Waste Fat by Dual-wavelength Spectrophotometry  

作　　者：LIU Jia-di XU Shu-jun XU Chun-xiang QIN Jin-ping 

机构地区：[1]College of Chemistry and Chemical Engineering,Nanjing University of Technology [2]Jiangsu Products Quality Services Inspection Testing Institute

出　　处：《Chinese Food Science》2012年第2期40-42,共3页中国食品科学（英文版）

基　　金：Supported by National Quality Inspection Bureau(KJ092102,2009QK146)

摘　　要：[Objective]The aim was to find a way to measure the cholesterol content in deserted oil.[Method]Dual-wavelength spectrophotometry method was used for the determination of cholesterol in edible oil.630 nm and 720 nm were set as target band and reference band in this method,respectively.The result was compared to liquid chromatography method.[Result]The relative standard deviation(RSD)was at 1.26% and recovery was between 101.9% and 110.7%.Linear range was 8.0-40.0 μg/ml.The tested result was close to the result of liquid chromatography.[Conclusion]It was proved that this method was easy,fast and accurate.The disturbance of sitosterol was eliminated without isolation.It can be used for fast identification of the authenticity of waste fat.[ Objective] The aim was to find a way to measure the cholesterol content in deserted oil. [ Method ] Dual-wavelength spectrophotometry method was used for the determination of cholesterol in edible oil. 630 nm and 720 nm were set as target band and reference band in this method, respectively. The result was compared to liquid chromatography method. [ Result] The relative standard deviation （RSD） was at 1.26% and recov-ery was between 101.9% and 110.7%. Linear range was 8.0 -40.0 μg/ml. The tested result was close to the result of liquid chromatography. [ Conclusion] It was proved that this method was easy, fast and accurate. The disturbance of sitosterol was eliminated without isolation. It can be used for fast identification of the authenticity of waste fat.

关 键 词：Dual-wavelength Spectrophotometry Waste fat CHOLESTEROL SITOSTEROL China 

分 类 号：O657.32[理学—分析化学] S816.17[理学—化学]