Screening of a Novel Bioflocculant-producing Strain and Research on Its Flocculation  

高絮凝活性菌株的筛选及其絮凝特性研究(英文)

作　　者：周爱华[1] 梁生康[2] 单宝田[3] 

机构地区：[1]青岛职业技术学院,山东青岛266555 [2]中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛266100 [3]中国海洋大学化学化工学院,山东青岛266100

出　　处：《Agricultural Science & Technology》2012年第9期1997-2000,2005,共5页农业科学与技术（英文版）

基　　金：Supported by the Science Research Project of Qingdao Technical College in 2012(12-A-2)~~

摘　　要：[Objective] This study aimed to screen a bacterial strain capable of producing bioflocculant. [Method] A bacterial strain T-11 capable of producing bioflocculant was isolated from activated sludge. Detailed tests on the morphological, physiological and biochemical characteristics were carried out and identification was performed to identify the strain. Finally, the bioflocculant was isolated and purified, and the flocculating activity and chemical characteristics were measured. [Result] It was identified as Serratia plumuthica based on its morphological, physiological and biochemical characteristics. This strain secreted flocculant best in a culture medium which included sucrose and NaNO3. The maximal cell growth was achieved within 10 h and the flocculating activity paralleled to it. It was found to be effective for flocculation of kaolin suspension, when added at a final concentration of 0.7 mg/L, over a range of pHs （2-7）, and temperature （approximately 30-80 ℃）. Chemical analysis indicated that the bioflocculant was an acidic polysaccharide consisting of glucose, glucuronic acid and galactose, talose and altrose. Infrared spectrum analysis also revealed typical characteristics of polysaccharides. [Conclusion] The biofloccu- lants produced by strain T-11 can greatly improve the ability of activated sludge to settle.[目的]筛选一株高絮凝活性菌株并探究其絮凝特性。[方法]从活性污泥中筛选出一株絮凝剂产生菌,并进行鉴定。对该菌株所产絮凝剂进行提取纯化,并研究其理化性质和絮凝特性。[结果]该菌株被鉴定为沙雷氏菌属(Serratiaplumuthica)。该菌在一定的培养条件下达到最高絮凝活性仅需10h,这有可能大大降低其生产成本。红外分析结果,该菌所产絮凝剂是一种酸性多糖。当微生物絮凝剂用量为0.7mg/L,pH为2-7,温度30-80℃时,均具有很高的絮凝活性。[结论]该菌株所产絮凝剂和其他研究的菌株相比,具有用量少和比较宽泛的pH、温度使用范围的特点。该菌株所产絮凝剂能大大提高活性污泥的沉降能力。

关 键 词：BIOFLOCCULANT Flocculating activity POLYSACCHARIDE Serratia plumuthica 

分 类 号：X172[环境科学与工程—环境科学] X703