EGCG对肝癌细胞株BEL-7402增殖凋亡及Stat3蛋白表达的影响  

Effects of EGCG on the proliferation and apoptosis and the expression of stat3 in BEL-7402 cells

作　　者：祁卫卫[1] 王营[2] 徐学军[3] 

机构地区：[1]徐州医学院,江苏徐州221002 [2]徐州医学院附属医院消化内科,江苏徐州221002 [3]郑州大学,河南郑州450052

出　　处：《商丘师范学院学报》2012年第9期79-82,共4页Journal of Shangqiu Normal University

摘　　要：研究了没食子儿茶素没食子酸酯(EGCG)对人肝癌细胞BEL-7402增殖凋亡及对信号转导蛋白和转录激活因子3(Stat3)蛋白及磷酸化Stat3蛋白表达的影响.应用MTT法检测不同浓度EGCG对BEL-7402细胞增殖的抑制作用,用流式细胞仪分析细胞的凋亡率,用ELISA方法检测EGCG对Stat3蛋白及磷酸化的Stat3蛋白表达的影响.结果表明:EGCG有抑制肝癌细胞增殖促进凋亡的作用,并呈剂量依赖性.ELISA结果显示EGCG能降低磷酸化Stat3蛋白的表达水平.EGCG能抑制肝癌细胞的增殖促进凋亡,其机制可能有与下调磷酸化的Stat3蛋白的表达有关.To study the effects of EGCG on the proliferation and apoptosis and the expression of Singnal transducer and activator of transcription - 3 （ Stat3 ） in human hepatocellular carcinoma cell line BEL - 7402. The effect of dif- ferent concentrations of GECG on the proliferation was detected by MTr assay. The Flow cytometric analysis was performed to detect the cell apoptosis . ELISA assay was applied to test the expression of Stat3 and phosphorylation Stat3. EGCG could inhibit proliferation and induce apoptosis in a dose dependent manner in BEL -7402 cells. Mo- reover , EGCG down - regulated the expression of phosphorylation Stat3. EGCG inhibits growth inhibition and in- duces apoptosis in human heptocellular carcinoma cell line BEL - 7402 possibly by clown - regulating p - Stat3 ex- pression.

关 键 词：EGCG BEL-7402细胞 增殖 凋亡 STAT3转录因子 

分 类 号：R735.7[医药卫生—肿瘤]