Ⅱ型抗癌晶体蛋白的芳香族氨基酸及其与抗肝癌活性间的关系  

Relationship Between Aromatic Amino Acids of Parasporin-2 and its Anti-Liver-Cancer Activity

作　　者：陈爱明[1] 林毅[1] 

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《华侨大学学报（自然科学版）》2012年第4期408-411,共4页Journal of Huaqiao University(Natural Science)

基　　金：教育部科学技术研究重点项目(211205);福建省自然科学基金资助项目(2011J01221);福建省高等学校新世纪优秀人才支持计划(2006年度)

摘　　要：利用氨基酸序列比对,蛋白质间相互作用位点预测和蛋白质与蛋白质对接,研究Ⅱ型抗癌晶体蛋白的氨基酸组成与其抗肝癌活性之间关系.结果表明:Ⅱ型抗癌晶体蛋白分子上位点49,51,52,55～60,194和205～212的氨基酸残基,特别是芳香族氨基酸在配体和受体蛋白质间的相互作用中起着重要作用;在Hex的蛋白质与蛋白质对接中,配体P11和P32与受体甘油醛-3-磷酸脱氢酶(GAPDH)的结合能量最低,表明P11和P32更容易与GAPDH结合.In this study, amino sequences blast, protein-protein interaction sites prediction and protein：protein docking were employed to explore the relationship between amino acid composition of parasporin-2 and its anti-liver-cancer activity. The results showed that amino residues, especially the aromatic amino residues at sites 49, 51, 52, 55-60, 194 and 205-212 in parasporin-2 were found to play a key role in ligand-reeeptor interaction. Protein-protein docking on Hex illustrated that ligands Pll and P32 had lower total binding energy value than the others when glyceraldehyde-3-phosphate dehydrogenase （GAPDH） was the receptor, indicated that Pll and P32 were easier to bind to GAPDH.

关 键 词：Ⅱ型抗癌晶体蛋白 苏云金芽孢杆菌 芳香族氨基酸 分子对接 甘油醛-3-磷酸脱氢酶 

分 类 号：Q511.04[生物学—生物化学] Q78