肉豆蔻挥发油中α-蒎烯,β-蒎烯含量测定及动态分析  

Determination and dynamic analysis of α-pinene and β-pinene in volatile oil of Myristica fragrans Houtt

作　　者：赵祥升[1] 黄立标[1] 杨新全[1] 冯锦东[1] 黄娴 

机构地区：[1]中国医学科学院 北京协和医学院药用植物研究所海南分所,万宁571533 [2]海南回元堂药业有限公司,海口570216

出　　处：《海峡药学》2012年第8期57-59,共3页Strait Pharmaceutical Journal

基　　金：海南省中药现代化专项课题(NO:2010ZY009);海南省重点科技计划项目(ZDXM20100009)

摘　　要：目的建立同时测定肉豆蔻挥发油中α-蒎烯和β-蒎烯含量的气相色谱法;研究肉豆蔻叶片挥发油中两种成分含量的动态变化。方法水蒸气蒸馏提取挥发油,GC法测定肉豆蔻挥发油中α-蒎烯和β-蒎烯的含量。气相色谱条件:HP-5MS石英毛细管柱(0.25mm×30m×0.25μm);氢火焰离子化检测器(FID);进样口温度240℃;检测器温度250℃;柱温起始温度为40℃,保持1min,以5℃.min-1的速率升高到220℃,保持1min;载气为氮气,流速1mL.min-1,分流比为10∶1,进样量为:1μL。结果α-蒎烯和β-蒎烯在相应的线性范围内呈现良好的线性关系,加样回收率(n=3)分别为:99.2%和98.7%。肉豆蔻不同组织挥发油中α-蒎烯和β-蒎烯含量有明显差异。不同月份肉豆蔻叶片挥发油中α-蒎烯和β-蒎烯含量变化呈现一定的规律性。结论方法简便快速,可以用于肉豆蔻挥发油中α-蒎烯和β-蒎烯的含量测定。OBJECTIVE To establish a GC method for simultaneous determination of α-pinene and β-pinene in volatile fractions and study its dynamic changes in leaves of Myristica fragrans Houtt;METHODS The essential oil was extracted by steam distillation.GC method was applied for the determination of α-pinene and β-pinene from the essential oil.Chromatographic conditions：HP-5MS Capillary column（0.25mm×30m×0.25μm） was used;The detector was FID;Inlet temperature was 240℃;The detector temperature was 250℃;Initial temperature was 40℃.It was programmed rising by 5℃·min-1 until 220℃.Carrying gas was nitrogen（1mL·min-1）,split ratio was 10：1;Injecton volume was 1μL.RESULTS α-pinene and β-pinene have good linear relationship in the concentration range with the average recoveries（n=3） were 99.2% and 98.7%,respectively.The contents of α-pinene and β-pinene in different tissues were different.The content of α-pinene and βpinene in the leaves from different months showed a certain degree of regularity.CONCLUSION This method is simple,quick and specific.It can be applied for the simultaneous determination of α-pinene and β-pinene in the volatile fractions of Myristica fragrans Houtt.

关 键 词：肉豆蔻 挥发油 Α-蒎烯 Β-蒎烯 气相色谱法 

分 类 号：R917[医药卫生—药物分析学]