四逆散中柴芍配伍对UC大鼠血清IL-6、IL-17的影响  

Influence of Compatibility of Bupleurum and Peony in Sini Powder on Expression of IL-6 and IL-17 in Ulcerative Colitis Rats

作　　者：卢健[1] 马骥[1] 陈金[1] 林庶茹[1] 

机构地区：[1]辽宁中医药大学方剂学科,辽宁沈阳110032

出　　处：《山西中医学院学报》2012年第3期31-32,共2页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：辽宁省教育厅项目(2006T094)

摘　　要：目的:探讨四逆散对实验性溃疡性结肠炎(UC)大鼠IL-6和IL-17的影响,以及柴芍君臣配伍在组方中的作用。方法:将实验动物分为正常组、模型组、阳性对照组、四逆散组、柴芍枳组、柴芍甘组和柴芍组7组,采用免疫法造模,用ELISA法检测大鼠结肠黏膜组织和血清IL-6、IL-17水平。结果:模型组大鼠血清IL-6、IL-17明显高于正常组(P<0.05);与模型组比较,阳性对照组、四逆散组、柴芍枳组和柴芍甘组大鼠血清IL-6、IL-17均显著降低(P<0.05),柴芍组血清IL-17明显降低;与四逆散组比较,柴芍枳组和柴芍组大鼠血清IL-6显著增高,柴芍甘组大鼠血清IL-17显著升高(P<0.05)。结论:四逆散能够降低UC大鼠血清IL-6、IL-17水平,方中柴芍的君臣配伍对IL-17的影响发挥了主要作用,但对IL-6的作用不明显,需配伍配伍甘草后才能发挥重要作用。Objective ：To study influence of compatibility of Bupleurum and Peony in Sini powders on expression of IL-6 and IL-17 in Ulcerative Colitis rats. Methods：The rats were randomly divided into normal group,model group,positive control group, Sini powder group, chaishaozhi group, chaishaogan group and chaishao group.Ulcerative Colitis model rats were induced by immunization method.IL-6 and IL-17 levels in colonic mucosal tissue and serum were detected with ELISA. Results： Compared with normal group, IL-6 and IL-17 levels in serum were increased in model group（P〈0.05 ）. Compared with model group, IL-6 and IL-17 levels in serum in positive control group, Sini powder group, chaishaozhi group and chaishaogan group were decreased,IL-17 level was decreased in chaishao group.Compared with Sini powder group,IL-6 level was increased in Chaishaozhi group and chaishao group （P〈O.05）,IL-17 level was increased in ehaishaogan group. Conclusion：Sini Powders can reduce the levels of IL-6 and IL-17 in UC rats. Compatibility of Bupleurum and Peony plays an important role in expression of IL-17.Compatibility of Bupleurum, Peony and licorice has effect on expression of IL-6.

关 键 词：四逆散 柴芍配伍 IL-6 IL-17 溃疡性结肠炎 

分 类 号：R574[医药卫生—消化系统]