蔗糖棕榈酸单酯的选择性酶促合成及其性能研究  

The characteristics of 6-O-palmitoylsucrose synthesized by selective enzymatic

作　　者：王萍[1] 杜理华[1] 何秀娟[1] 吴石金[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2012年第5期488-492,共5页Journal of Zhejiang University of Technology

基　　金：国家自然科学基金资助项目(20977087);浙江省自然科学基金资助项目(Y5090054)

摘　　要：研究了脂肪酶生物催化法选择性合成蔗糖-6-棕榈酸酯及其性能.蔗糖-6-棕榈酸酯的转化率为94%,产物的结构经1 H-NMR确证.测定了产物蔗糖-6-棕榈酸酯的水溶性、亲水亲油平衡值(HLB)、表面张力(CMC)以及泡沫性能和乳化性能等.结果显示其具有很好的水溶性,HLB=16.11,CMC=24.8mg/L,γCMC=35.78mN/m,泡沫和乳化性能在实验浓度范围内呈一定的规律.抑菌试验表明该蔗糖-6-棕榈酸酯对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌均无明显抑菌效果.The properties of 6-O-palmitoylsucrose biocatalysized by lipase were studied. The 6-O- palmitoylsucrose purified by column chromatography had a higher percentage of monoesters （94%）. The 6-O-palmitoylsucrose was identified by 1^H-NMR techniques. The surfactant properties of 6-O-palmitoylsucrose which contains the hydrophilic-lipophilic balance（HLB）, foam properties, emulsion properties and the critical micelle concentration（CMC） were determined. The result showed that the 6-O-palmitoylsucrose has good solubility in water with the hydrophilic-lipophilic balance （HLB） value of 16. 11. The result of determination of surface tension is CMC=24.8 mg/L, YCMC= 35. 78 mN/m by Du Nouy. The foam and emulsion show a certain regularity in the full range of experimental concentrations. The antimicrobial activities of 6-O-palmitoylsucrose were tested by Escherichia coli, Staphylococcus aureus and Bacillus subtilis. , and the result showed that the monoester had no antibacterial activity apparently.

关 键 词：蔗糖-6-棕榈酸酯 生物催化 脂肪酶 性能 

分 类 号：TQ423.92[化学工程]