CD117、CD34、DOG-1、WT-1在胃肠道间质瘤中的表达及意义  

Expression and Significance of DOG-1,CD117,CD34 and WT-1 in Gastrointestinal Stromal Tumors

作　　者：李红玲[1] 易红梅[1] 李艳春[1] 陈志鸿[1] 刘宇[1] 

机构地区：[1]湖南省人民医院病理科,湖南长沙410005

出　　处：《现代生物医学进展》2012年第26期5064-5067,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨DOG-1、CD117、CD34、WT-1在胃肠道间质瘤(GIST)中的表达及临床意义。方法:应用免疫组织化学SP法检测DOG-1、CD117、CD34、WT-1在39例GIST患者肿瘤组织中的表达。结果:GIST光镜下主要由梭形细胞和(或)上皮样细胞或多形性细胞混合或单一性组成。DOG-1、CD117、CD34和WT-1在GIST肿瘤组织中阳性表达率分别为92.3%(36/39)、71.7%(28/39)、64.1%(25/39)、23.1%(9/39),四者的阳性表达率在各风险程度组(极低及低度危险性GIST、中度危险性GIST、高度危险性GIST)两两比较中差异均无统计学意义(P>0.05)。DOG-1与CD117相比,在极低及低度危险组中表达有显著差异(P<0.05),DOG-1与CD34相比,在极低及低度危险组、高度危险组中表达有显著差异(P<0.05);WT-1与CD117相比,在中度及高度危险组中表达有显著差异(P<0.05),WT-1与CD34相比,在中度危险组中表达有显著差异(P<0.05)。对照组平滑肌瘤、纤维瘤、神经鞘瘤中CD117、DOG-1在GIST中的表达明显高于其他多种梭形细胞的表达(P<0.05)。结论:DOG1是GIST较为敏感和特异的标记物,WT-1在极低及低度危险性GIST中有一定的诊断价值,在GIST的诊断中加入两者将使患者更加受益。Objective： To investigate the expression of DOG-1,CD117,CD34 and WT-1 in gastrointestinal stromal tumors（GIST） and its diagnostic application. Methods： Immunohistochemical SP technique was used to assess the expression of DOG-1,CD 117,CD34 and WT-1 in 39 cases of GIST. Results： All 39 cases of GIST consisted of variable proportion of spindle and epithelioid tumor cells or just one type of the tumor cell. The expression rates of DOG-1, CD117, CD34 and WT-1 were 92.3%（36/39）, 71.7%（28/39）, 64.1% （25/39）, 23.1%（9/39）. The expression rates of DOG-1, CD117, CD34 and WT-1 have no statistical difference in the group of very low and low risk GIST,moderate risk GIST,high risk GIST （P 〉0.05 ）, The sensitivity of DOG-1 detection of very low and low risk GIST were significantly higher than those of CD117 （P〈0.05）, The sensitivity of DOG-1 detection of very low and low risk GIST, high risk GIST were significantly higher than those of CD34（P〈0.05 ）; The sensitivity of WT-1 detection of moderate risk and high risk GIST were significantly higher than those of CD 117 （P〈0.05）, The sensitivity of WT-1 detection of moderate risk GIST were significantly higher than those of CD34 （P〈0.05）. The expression of DOG-1 and CD 117 in GIST were much higher than leiomyomas,schwannomas,fibromas （P〈0.05）. Conclusion： DOG-1 is a sensitive and specific marker for GIST; WT-1 has some reference value in the diagnosis of very low and low risk GIST; more patients will benefit from the joining of DOG-1 and WT-1.

关 键 词：胃肠道间质瘤 DOG-1 WT-1 CD117 CD34 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R735.3[医药卫生—临床医学]