Her2(+)/Her2(-)乳腺癌差异表达microRNAs的生物信息学分析  

Bioinformatics-based analysis for differently expressed microRNAs between Her(+)/Her(-) breast cancers

作　　者：曾融[1] 张积仁[1] 姜茂竹[1] 麦仲伦[1] 吴钢[1] 郑燕芳[1] 

机构地区：[1]南方医科大学珠江医院肿瘤中心,广州510282

出　　处：《山东医药》2012年第33期5-8,共4页Shandong Medical Journal

基　　金：卫生部科技专项基金(W2009BX015)

摘　　要：目的通过文献挖掘与生物信息学分析的方法,探讨Her2(+)/Her2(-)乳腺癌之间差异表达的microRNAs可能涉及的生物学功能和信号通路。方法在NCBI数据库的GEO子数据库及EBI数据库中的ArrayEx-press子数据库开展检索,使用关键词"breast cancer"、"microRNA"进行检索,获取Her2(+)/Her2(-)乳腺癌之间差异表达microRNAs。利用microRNAs靶基因预测软件预测所有差异表达microRNAs的靶基因,进行通路分析。结果找到2个在Her2表型不同的乳腺癌中差异表达的microRNAs数据集,利用软件TargetScan预测差异表达microRNAs的靶向基因,取其合集进行富集分析,最后将富集得到的基因利用David数据库进行分析,从而得到了上述差异表达的microRNAs可能具有的生物学功能和参与的调节通路。结论差异表达的microRNAs通过调节靶向基因参与不同信号通路,实现多种生物学功能。Objective To explore biological functions and pathways of differentially expressed miRNAs between different Her2 phenotypes of breast cancer through literature mining and bioinformatic analysis. Methods Carrying out the search in GEO, the sub-database of the NCBI database and Array Express, the sub-database of the EBI database, using key words ＂breast cancer miRNA＂ to determine the useful literature. According to literature provided by the Pmicroarray data, Her2 （ ＋ ） / （ - ） breast cancer difference between the expression of miRNAs was found. Finally, using miRNAs target gene prediction softwares to predict all the differentially expressed miRNAs target genes and carry out pathway analysis. Results Two microRNAs collections differentially expressed in Her2 phenotypes of different breast cancers, then using softwares TargetScan to finish enrichment analysis, and finally using David database to enrich gene analysis, which may have biological functions of these differentially expressed microRNAs and participate in the pathway regulating. Conclusion The differentially expressed microRNAs regulate target genes involves in different signaling pathways to achieve a variety of biological functions.

关 键 词：乳腺肿瘤 受体 Her2 小RNA病毒 计算机生物学 

分 类 号：R737.9[医药卫生—肿瘤]