人乳头瘤病毒和单纯疱疹病毒与宫颈癌的关系  

The relationship between human papillomavirus and herpes simplex virus and cervical carcinoma

作　　者：宋连芳[1] 

机构地区：[1]山东省苍山县人民医院,山东苍山277700

出　　处：《医学检验与临床》2012年第4期25-26,共2页Medical Laboratory Science and Clinics

摘　　要：目的探讨人乳头瘤病毒（HPv）和单纯疱疹病毒（HSV）等对宫颈癌的病因学作用。方法应用聚合酶链反应（PCR）-核酸内切酶分型检测宫颈癌活检组织中HPV—DNA和HSV—DNA基因，以正常宫颈组织作对照。结果在宫颈癌活检组织中HPV-16，18，35型和HSV—Ⅱ型阳性率分别为38．9％争36．2％，与正常妇女宫颈组织阳性率均为3．2％比较，差异均有非常显著意义（P〈0．001）。结论HPV-16，18型和HSV-Ⅱ型是诱发宫颈癌的重要病原因子。Objective This study was to detect the human papillomavirus （HPV） and herpes simplex virus （HSV） genotypes in biopsy specimens of cervical Carcinoma and genital verruca by PCR - endonuclease cleavage. Methods Biopsy samples of were obtained from 240 pa- tients with cervical carcinoma, HPV and HSV genotypes were detected by PCR and endonuclease cleavage. Results The results indicated that HPV types 16, 18, 35 were found in 93 （38.9%） of 240 cervical carcinoma, in 85（31.7% ） of 268 cervical erosion, while HPV types 6, 11 were found in 51 （59.3 % ） of 86 acuminate erruca. The HPV 16, 18 types and HSV - Ⅱ type found in normal cervical secretions of the con- trul group all were 3.2%, There was significant difference between the test group of cervical carcinoma and the control group of normal cervical secretion （P〈 0.001）. Conclusion The results-suggest that HPV 16, 18 ,35types and HSV -Ⅱ type are associated with majority of the cer- vical carcinoma.

关 键 词：宫颈癌 人乳头瘤病毒 单纯疱疹病毒 PCR荧光探针法 

分 类 号：R772.210.5[医药卫生—眼科]