槲皮素修饰玻碳电极(Qu/GCE)的制备及铜离子-牛血清白蛋白结合反应机制研究  

Investigation of the binding interaction between copper ions and bovine serum albumin on Quercetin-modified glassy carbon electrode

作　　者：郭明[1] 何玲[1] 孙一新[1] 关颖[1] 李铭慧[1] 

机构地区：[1]浙江农林大学化学系,临安311300

出　　处：《环境化学》2012年第9期1355-1361,共7页Environmental Chemistry

基　　金：国家自然科学基金(20877072);浙江省科技创新项目(2010R412042)资助

摘　　要：通过滴涂法制备槲皮素修饰玻碳电极(Qu/GCE,Quercetin/Glassy Carbon Electrode),并对修饰电极进行电化学性能表征;利用槲皮素修饰电极研究了Cu2+离子与牛血清白蛋白(BSA,Bovine Serum Albumin)的结合反应机制.循环伏安法测试结果显示该修饰电极具有准可逆氧化还原反应;将槲皮素修饰电极应用于测试重金属离子-血清白蛋白的结合反应机制,在pH 5.0 HAc-NaAc缓冲溶液体系中,使用槲皮素修饰电极测定Cu2+-BSA相互作用,显示出良好的线性响应关系.根据文献报道结合实验数据,本文建议了结合参数计算理论方程,并利用建议的理论方程计算了Cu2+-BSA的结合常数及结合位点数,相关研究结果可为槲皮素修饰电极研究非共价相互作用提供有益参考.A Quercetin modified glassy carbon electrode was prepared by drop-dry method,and its electrochemical properties were characterized.The interaction between Cu2＋ ions and bovine serum albumin（BSA） was studied on the Quercetin modified electrode.The results showed that the Quercetin modified electrode exhibited quasi-reversible redox reaction by cyclic voltammetry.The modified electrode was used to study the binding interaction between Cu2＋ and BSA in pH 5.0 NaAc-HAc buffer solution.A theoretical treatment was proposed to calculate the binding parameters of the reaction.The binding constant and number of binding sites of the interactions were calculated to be 1.93×105 L · mol-1 and 1.10,respectively.The results provide a method to study noncovalent interaction on the Quercetin modified electrode.

关 键 词：修饰电极 槲皮素 CU2+ 牛血清白蛋白 结合反应机制 

分 类 号：X171.5[环境科学与工程—环境科学]