BRMS1基因对人直肠癌裸鼠成瘤性及转移能力的影响  

Effects of BRMS1 on tumorigenicity and metastasis of human rectal cancer cells in nude mice

作　　者：孙燕来[1] 宋希林[2] 唐鲁兵[3] 王斌[1] 张毅[1] 

机构地区：[1]山东省肿瘤医院外科四病区,济南250117 [2]山东省肿瘤医院外科八病区,济南250117 [3]宁波市妇女儿童医院乳腺外科,浙江宁波315012

出　　处：《山东大学学报（医学版）》2012年第9期25-28,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(Y2007c118);山东省医学科学院院级科技计划(2010-10)

摘　　要：目的探讨转移抑制BRMS1基因对人直肠癌裸鼠成瘤性以及抑制肿瘤细胞远处转移的影响。方法通过将BRMS1基因导入人直肠癌细胞株LOVO中,建立裸鼠实验性转移模型和自发性转移模型,观察转染组(A组)、空载体组(B组)、未转染组(C组)裸鼠的生长状态和肿瘤远处转移状况。结果自发性转移模型:A1组转染组细胞、B1组转染空载体组细胞和C1组转染组癌细胞接种后裸鼠的体质量及肿瘤平均直径无统计学差异(P>0.05)。实验性转移模型:转染空载体组B2和未转染组C2的裸鼠肺脏转移率为100%;转染组A2组肺脏和肝脏转移灶的病理分级标准较B2组和C2组降低,A2组肺转移分级以II级为主,B2组、C2组以II级、III级为主;A2组未见肝转移灶,而B2组、C2组肝转移分级以I级、II级为主。结论 BRMS1基因能够明显抑制直肠癌LOVO细胞的远处转移的能力,但并不影响肿瘤细胞本身的生长,提示BRMS1基因可能成为抑制直肠癌远处转移的新靶点。Objective To investigate the effects of BRMS1 on tumorigenicity and inhibition for the distant metastasis of implanted human rectal carcinoma in nude mice.Methods The experimental transplanted and spontaneous transplanted models were constructed in nude mice by transferring BRMS1 gene into human rectal cancer cell line LOVO.Growth of nude mice and distant metastasis of cancer cells in transfected group（A）,non-carrier group（B） and non-transfected group（c） were observed.Results The spontaneous transplanted model ： there were no significant differences on the average diameter of inoculated tumor and the body mass among groups A1,B1 and C1.The experimental transplanted model ： the pulmonary metastasis rates were 100% in groups B2 and C2,but diffuse infiltration was the main form in pulmonary tissues;the pathology degrees of pulmonary and hepatic metastasis of group A2 were slightly decreased compared with groups B2 and C2;the pulmonary metastasis degree of group A2 was mainly grade Ⅱ,and mainly grades Ⅱ and Ⅲ in groups B2 and C2;liver metastasis was not detected in group A2;the pathology degrees of liver metastasis in groups B2 and C2 were mainly grades Ⅰ and Ⅱ.Conclusion BRMS1 can inhibit the distant metastasis of rectal carcinoma LOVO cells,but has no effect on the tumor growth,which suggests that the BRMS1 gene could be a new target for the inhibition on the colorectal cancer distant metastasis.

关 键 词：乳腺癌转移抑制基因-1 直肠肿瘤 基因治疗 远处转移 转移抑制基因 

分 类 号：R73-37[医药卫生—肿瘤]