伊班膦酸钠在正畸源性根吸收中对破骨细胞的影响  

Effects of ibandronate on osteoclast in orthodontic root resorption

作　　者：丛淑敏 王旭霞[2] 张丽娜[2] 李涛[2] 王胜林[3] 李静[2] 张君[2,4] 

机构地区：[1]山东省警官总医院口腔科,济南250002 [2]山东大学口腔医学院,济南250012 [3]淄博市中心医院口腔科,山东淄博255000 [4]山东省口腔生物医学重点实验室,济南250012

出　　处：《山东大学学报（医学版）》2012年第9期44-49,共6页Journal of Shandong University:Health Sciences

基　　金：山东省科学技术发展计划项目(2009GG20002042);淄博市科技局科技攻关项目(2010GG01070)

摘　　要：目的观察牙齿移动过程中破牙骨质细胞和破骨细胞分化因子(ODF)表达的变化,研究伊班膦酸钠对大鼠正畸源性根吸收的作用。方法选取48只SPF级雌性Wistar大鼠,建立正畸牙齿移动动物模型,并设置自身对照。所有大鼠均于安装矫治器前3天在实验侧牙齿近中腭侧黏骨膜下局部注射50μL伊班膦酸钠,对照侧注射50μL生理盐水。每3天注射一次至实验结束。实验第3、7、14天各处死16只大鼠,随机选择8只作组织学切片观察,另外8只作扫描电镜(SEM)观察。结果①实验第7天和14天实验侧破牙骨质细胞数量小于对照侧(P<0.05);②实验第7天和第14天实验侧牙周组织压力侧ODF阳性表达程度低于对照侧(P<0.05);③SEM结果显示,实验侧第3、7和14天牙根表面吸收陷窝较对照侧数量少、面积小。结论局部应用伊班膦酸钠可以减少牙周组织压力侧破牙骨质细胞的数量,降低牙周组织压力侧ODF阳性表达程度,减少正畸源性根吸收的发生。Objective To investigate the influence of ibandronate on orthodontic tooth resorption in rats through observing the histological changes,cementoclasts in periodontal tissues and the expression of osteoclast differentiation factor（ODF）.Methods A total of 48 SPF female Wistar rats were chosen in this study,and the rat model for orthodontic tooth movement was established.50 μL ibandronate solution was injected into the sub-periosteum area adjacent to the left upper first molar（the experimental side） every 3 days during the experiment,and the right maxillary first molar was served as a control（the control side） with an injection of 50 μL 0.9% NaCl solution into the corresponding area at the same time intervals.The administration of ibandronate solution was started 3 days before the setting of appliances.After orthodontic force was applied for 3,7 and 14 days,the rats were killed in batches（16 animals in each period）.8 rats were chosen for histology observation of periodontal tissues,and the remaining 8 were used for scanning electron microscopy（SEM） observation of root resorption.Results ① On days 7 and 14,the number of cementoclasts in the experimental side was significantly less than in the control side（P0.05）.② The expression of ODF on the pressure side of periodontium on experimental upper first molar was significantly lower than that on the control side on days 7 and 14（P0.05）.③ The SEM results showed that on days 3,7 and 14,there were fewer and smaller resorption lacunae in the experimental side than in the control side.Conclusion This study demonstrateed that local administration of ibandronate can reduce the number of osteoclasts in the pressure side of periodontal tissues,lower the expression of ODF,and inhibit orthodontic root resorption.

关 键 词：伊班膦酸钠 破牙骨质细胞 牙根吸收 破骨细胞分化因子 扫描电镜 

分 类 号：R783.5[医药卫生—口腔医学]