SATB1与HER2表达和乳腺癌分化程度的相关性  

Correlation between SATB1 and HER2 expressions and the tumor grade in breast cancer

作　　者：王婧男[1] 郑燕[2] 肖东杰[2] 李金星[1] 丁卜同[2] 刘相东[3] 汪运山[2,4] 

机构地区：[1]山东中医药大学附属医院检验科,济南250013 [2]山东大学附属济南市中心医院中心实验室,济南250013 [3]山东大学附属省立医院检验科,济南250021 [4]山东大学附属济南市中心医院医学实验诊断中心,济南250013

出　　处：《山东大学学报（医学版）》2012年第9期91-95,104,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81000869);山东省优秀中青年科学家科研奖励基金(BS2011YY039);济南"泉城学者"建设工程招标课题(Q2-06)

摘　　要：目的检测特异性核基质结合蛋白(SATB1)和人表皮生长因子受体2(HER2)在乳腺癌中的表达,探讨其与乳腺癌分化程度的关系。方法应用免疫组织化学方法检测乳腺癌患者SATB1和HER2蛋白的表达,使用荧光原位杂交(FISH)方法检测HER2基因扩增状态,分析二者表达的相关性及与临床病理参数之间的关系。结果乳腺癌中存在SATB1与HER2的高表达,且二者呈正相关(r=0.425,P<0.05)。SATB1和HER2的表达与肿瘤组织分级有关,其共表达的肿瘤组织具有更高的组织学分级,与Ⅲ级乳腺癌相关(r=0.472,P<0.05)。结论SATB1可调节乳腺癌中HER2的表达,SATB1/HER2双阳性表达是乳腺癌低分化的一个标志。Objective To test the expressions of special AT rich sequence binding protein（SATB1） and human epidermal growth factor receptor 2（HER2）,and to investigate their relationships with the differentiation of breast carcinoma.Methods Expressions of SATB1 and HER2 were evaluated by immunohistochemistry method.Fluorescence in situ hybridization（FISH） was used to test the HER2 gene amplification.The association between SATB1 and HER2 expressions and their relationships with the clinicopathologic features of breast carcinoma were analyzed.Results SATB1 and HER2 were highly expressed in breast carcinoma,and there were a significant correlation between the two（r=0.425,P0.05）.SATB1 and HER2 expressions were related to the tumor histological grade,and the histological grade of double positive samples was higher.Co-expression of them was significantly correlated with tumor grade Ⅲ in breast cancer（r=0.472,P0.05）.Conclusion SATB1 could regulate the expression of HER2,and SATB1/HER2 positive co-expression might be a poorly differentiated marker in the breast cancer.

关 键 词：乳腺肿瘤 SATB1蛋白 HER2蛋白 组织分级 低分化 荧光原位杂交 

分 类 号：R737.9[医药卫生—肿瘤]