多西紫杉醇诱导前列腺癌PC3细胞凋亡的蛋白质组学研究  

A proteomics study of docetaxel-induced apoptosis in prostate cancer PC3 cells

作　　者：萧畔[1] 张怀强[1] 崔亚洲[2] 赵升田[1] 马天加[1] 周春文[1] 

机构地区：[1]山东大学第二医院泌尿外科,济南250033 [2]山东医药生物技术研究中心山东省医学科学院,济南250062

出　　处：《山东大学学报（医学版）》2012年第10期77-80,85,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(Z2007C07)

摘　　要：目的比较多西紫杉醇诱导激素非依赖性前列腺癌PC3细胞凋亡前后的差异表达蛋白。方法采用磺酰罗丹明B(SRB)法检测不同浓度多西紫杉醇对前列腺癌PC3细胞增殖的抑制作用;应用胶内差异凝胶电泳对PC3细胞凋亡前后蛋白质进行双向凝胶电泳图谱分析,分离并筛选鉴定相关差异蛋白。结果半数抑制浓度IC50为20 nmol/L。双向凝胶电泳图谱发现表达差异较为明显的18个蛋白质位点,其中8个蛋白表达上调,10个蛋白表达下调。结论本研究发现的部分差异蛋白可能参与激素非依赖性前列腺癌对紫杉醇类药物的耐药作用。Objective To investigate the preliminary molecule mechanism of docetaxel resistance in prostate cancer cells by comparing the differential protein expressions before and after docetaxel-induced apoptosis in androgen inde- pendent prostate cancer PC3 cells. Methods The sulforhodamine B method was used to detect the inhibition effect of different concentrations of docetaxel on the proliferation of PC3 cells. Differential gel electrophoresis （DIGE） was a- dopted to separate and identify the differential proteins before and after docetaxel-induced apoptosis. Results The me- dian inhibitory concentration IC50 was 20 nmol/L. Eighteen significant differential protein spots were defined by DIGE. Eight proteins were up-regulated while ten proteins were down-regulated. Conclusion Eighteen significant differential protein spots were identified by using the proteomics method. It was believded that various differential proteins might be involved in the docetaxel resistance in androgen independent prostate cancer therapy.

关 键 词：前列腺肿瘤 紫杉醇 蛋白质组学 差异凝胶电泳 细胞凋亡 

分 类 号：R737.25[医药卫生—肿瘤]