精子蛋白17靶向的小鼠肿瘤近红外荧光成像  

Sperm protein 17 targeted near-infrared fluorescence imaging of tumor in mice

作　　者：李芳秋[1] 张士新[1] 安联校[2] 顾月清[2] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所,南京理学博士210002 [2]中国药科大学生命科学学院,南京210009

出　　处：《医学研究生学报》2012年第9期901-904,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金(30670599);南京军区医学科学技术"十一五"研究计划重点项目(06Z43)

摘　　要：目的人精子蛋白17(human sperm protein 17,Sp17)异常表达在一些恶性肿瘤细胞,可被用作肿瘤特异性分子诊断和治疗的靶点。文中将抗Sp17单克隆抗体与近红外染料偶联,制成高亲和力探针,用近红外成像技术对活体动物肿瘤靶点进行确认。方法用免疫组化技术证明,Sp17在肝细胞癌细胞系SMMC-7721及裸鼠皮下移植瘤组织高水平异常表达。将近红外染料吲哚花箐素衍生物(ICG-Der-02)与抗Sp17单克隆抗体(anti-Sp17 mAb)偶联,获得特异性探针。静脉注射至荷瘤小鼠体内,用光学成像系统进行活体实时肿瘤显像。结果 anti-Sp17-ICG-Der-02探针进入动物体内后,快速聚集到肿瘤部位,发出强烈的荧光信号。随着未结合染料逐渐排出体外,动物肝、肾等内脏器官的非特异荧光信号消褪,清晰显示出肿瘤的部位及大小,且可以持续至少7d。结论高亲和力探针anti-Sp17-ICG-Der-02对体内肿瘤特异性诊断有潜在应用价值。Objective Human sperm protein 17 （Sp17） is aberrantly expressed in some cancers, and can be used as a target for specific molecular diagnosis and treatment of these malignancies. The purpose of this study is to identify tumors in mice with a high- affinity probe consisting of a near-infrared （NIR） and the specific monoclonal antibody against Sp17 （anti-Sp17-mab）. Methods We used immunohistochemistry to identify the highly abnormal expression of Sp17 in the hepatocellular carcinoma cell line and tumor xenografts in mice. By coupling a NIR fluorescent （NIRF） cyanine dye （indocyanine green derivative 2, ICG-Der-02） to anti-Sp17-mab, we designed a bio-optical high-affinity fluorescent probe anti-Sp17-ICG-Der-02, which was injected into tumor-beating nude mice through the caudal vein for evaluating its tumor-targeting effect by the near infrared imaging system. Results The overexpression of Sp17 was demonstrated on the surface of the hepatocellular carcinoma cell line SMMC-7721. Anti-Sp17-ICG-Der-02 with immuno-activity was successfully synthesized. The immuno-activity and photo stability of anti-Sp17-ICG-Der-02 exhibited a high targeting capability in Sp17 expressing tumor models （ SMMC-7721 ）, and its accumulation in tumors lasted for at least 7 days. Conclusion The high-affinity proble anti- Sp17-ICG-Der-02 has a potential application value for the specific diagnosis of tumor in vivo.

关 键 词：精子蛋白17 靶向探针 肿瘤 光学成像 

分 类 号：R730.43[医药卫生—肿瘤]