HPLC法测定藏成药八味小檗皮散中没食子酸含量  

Determination of gallic acid in Bawei Xiaobopi powder by HPLC

作　　者：张力力[1] 杨晓燕[1] 张琦[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2012年第5期780-784,共5页Journal of Southwest Minzu University(Natural Science Edition)

摘　　要：目的:建立八味小檗皮散中没食子酸的含量测定方法.方法:采用高效液相色谱法,色谱柱:SwellChromstarTMC18柱(5μm,4.6×250mm);流动相:甲醇:水:磷酸(5:95:0.05);流速:1ml/min;检测波长:280nm;柱温:30℃.结果:没食子酸在24.1μg/ml～144.6μg/ml范围内呈良好的线性关系,回归方程为A=12.574C+6.251,r=0.9998;平均加样回收率为98.56%,RSD为2.24%.结论:该法简便可靠,能准确测定八味小檗皮散中没食子酸的含量,适用于八味小檗皮散质量标准的提高.： Objective： To establish an HPLC method for the determination of gallic acid in BaweiXiaobopi powder. Method： HPLC method is used; The column is Swell ChromstarTM C18 （5μm, 4.6×250mm） and the mobile phase is Methanol-waterphosphate （5：95：0.05）; The flow rate is 1ml/min; Contents are detected at a wavelength of 280nm; The column temperature is 30℃. Results： Gallic acid shows a good linear relationship in 24.1μg/ml -144.6μg/ml and the regression equation is A= 12.574C＋6.251, r = 0.9998; The average recovery rat is 98.56% and the RSD is 2.24%. Conclusion： The method is simple, reliable, and accurate for determination of gallic acid in BaweiXiaobopi powder, which is applicable for the improvement of the quality standards of Bawei Xiaobopi powder.

关 键 词：八味小檗皮散 没食子酸 高效液相色谱法 

分 类 号：R284[医药卫生—中药学] R291.408[医药卫生—中医学]