免疫杀伤细胞治疗对恶性肿瘤患者T淋巴细胞免疫功能的影响  

The impact of cascade primed immune cell therapy on the immune function of T lymphocytes in patientswith malignant tumor

作　　者：阮月芹[1] 韩晓通 王敏[2] 李卫[2] 韩兆东[3] 

机构地区：[1]山东省滨州医学院附属医院检验科,256603 [2]山东省滨州医学院附属医院中心实验室,256603 [3]黑龙江中医药大学

出　　处：《中华生物医学工程杂志》2012年第4期292-295,共4页Chinese Journal of Biomedical Engineering

摘　　要：目的探讨链式激活和诱导的免疫杀伤细胞（CAPRI细胞）治疗对肿瘤患者T淋巴细胞免疫功能的影响。方法接受CAPRI细胞治疗的滨州医学院附属医院肿瘤内科收治的50例恶性肿瘤患者为研究对象（肿瘤组），同时选择体检健康者30例为对照组，用流式细胞术检测所有患者治疗前后外周血T淋巴细胞CD3±及CD4^＋、CD8^＋的百分率，计算CD4^＋／CD8^＋比值并进行比较。结果肿瘤患者治疗前外周血CD3^＋、CD4^＋、CD8±、CD4±／CD8^＋[（56．40±7．14）％、（30．69±8．01）％、（29．10±7．13）％、1．05±0．28]与健康对照组[（66．78±6．05）％、（41．35±5．71）％、（26．08±5．36）％、1．59±0．31]及治疗后13d[（59．35±6．31）％、（34．95±7．21）％、（27．95±6．03）％、1．25±0．22]和治疗后25d[（62．36±6．41）％、（37．03±7．62）％、（25．04±4．53）％、1．46±0．48]比较，CD3^＋、CD4^＋、CD4^＋／CD8^＋均明显降低、CD8±均明显升高（均P〈0．05）；治疗后25d与治疗后13d比较，CD3^±、CD4^±、CD4^±／CD8^±均明显升高、CD8±均明显降低（均P〈0．05）。结论CAPRI细胞治疗肿瘤，能够纠正CD3^＋、CD4^＋、CD8^＋、CD4^＋／CD8^＋失衡状态，能够调节机体T淋巴细胞的免疫功能。Objective To explore the impact of cascade primed immune（CAPRI） cell therapy on the immune function of T lymphocytes in patients with malignant tumors. Methods A total of 50 cases of malignant tumors （tumor group） received CAPRI cell therapy in Department of Tumor in hospital and 30 healthy people as controls （control group） were enrolled in the study. The percentages of CD3^＋, CD4^＋ and CD8^＋ T lymphocytes were detected in peripheral blood by flow cytometry in all patients before and after treatment, then the ratio of CD4 ＋/CD8 ＋ T lymphocytes was calculated and compared. Results Before treatment, the percentages of CD3^＋, CD4^＋, CD8^＋ and CD4^＋/CD8＋ T lymphocytes in peripheral blood were （56.40±7.14） %, （ 30.69±8.01 ） %, （ 29.10±7.13 ） % and 1.05±0.28 in tumor group, which were significantly decreased in CD3＋ , CD4 ＋ , CD4 ＋/CD8 ＋ hut increased in CD8＋ as compared with healthy control group [ （66.78±6.05, 41.35±5.71, 26.08±5.36）%, 1.59±0.31], 13 d after treatment[ （59.35±6.31, 34.95±7.21, 27.95＋6.03）%, 1.25±0.22] and 25 d after treatment[ （62.36±6.41, 37.03±7.62, 25.04±4.53）%, 1.46± 0.481 , all P〈0.05]. After treatment, the percentages of CD3＋, CD4＋ and CD4＋/CD8＋ were significantly increased but CD8＋ decreased in tumor group at 25 d as compared with 13 d（all P〈0.05）. Conclusion CAPRI cell therapy for tumor can rectify the imbalance of CD3 ＋ , CD4 ＋ , CD8 ＋ and CD4 ＋/CD8 ＋ T lymphocytes, which can regulate the immune function of T lymphocytes.

关 键 词：杀伤细胞 淋巴因子激活 肿瘤 T淋巴细胞 杀伤细胞 

分 类 号：R730.5[医药卫生—肿瘤]