Isolation,identification and characterization of an algicidal bacterium from Lake Taihu and preliminary studies on its algicidal compounds  

作　　者：Chuan Tian Xianglong Liu Jing Tan Shengqin Lin Daotang Li Hong Yang 

机构地区：[1]State Key Laboratory of Microbial metabolism,School of Life Science and Biotechnology,Shanghai Jiao Tong University,800 Dongchuan Rd.Shanghai 200240,China

出　　处：《Journal of Environmental Sciences》2012年第10期1823-1831,共9页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 20877055);the National Basic Research Program (973) of China (No. 2012CB720802);the National High Technology Research and Development Program (863) of China (No. 2011AA100901)

摘　　要：In an effort to identify a bio-agent capable of controlling cyanobacterial blooms, we isolated a bacterial strain, A27, which exhibited strong algicidal activity against the dominant bloom-forming species of Microcystis aeruginosa in Lake Taihu. Based on 16S rRNA gene sequence analysis, this strain belongs to the genus Exiguobacterium. This is the first report of an algicidal bacterial strain belonging to the genus Exiguobacterium. Strain A27 exhibited algicidal activity against a broad range of cyanobacteria, but elicited little or no algicidal activity against the two green algal strains tested. The algicidal activity of strain A27 was shown to be dependent on the density of the bacteria and to have a threshold density of 1.5×106 CFU/mL. Our data also showed that the algicidal activity of strain A27 depended on different growth stages ofMicrocystis aeruginosa （exponential ≈ lag phase 〉 early stationary） rather than that of the bacterium itself. Our results also suggested the algicidal activity of strain A27 occurred via the production of extracellular algicidal compounds. Investigation of the algicidal compounds revealed that there were at least two different algicidal compounds produced by strain A27. These results indicated that strain A27 has great potential for use in the control of outbreaks of cyanobacterial blooms in Lake Taihu.In an effort to identify a bio-agent capable of controlling cyanobacterial blooms, we isolated a bacterial strain, A27, which exhibited strong algicidal activity against the dominant bloom-forming species of Microcystis aeruginosa in Lake Taihu. Based on 16S rRNA gene sequence analysis, this strain belongs to the genus Exiguobacterium. This is the first report of an algicidal bacterial strain belonging to the genus Exiguobacterium. Strain A27 exhibited algicidal activity against a broad range of cyanobacteria, but elicited little or no algicidal activity against the two green algal strains tested. The algicidal activity of strain A27 was shown to be dependent on the density of the bacteria and to have a threshold density of 1.5×106 CFU/mL. Our data also showed that the algicidal activity of strain A27 depended on different growth stages ofMicrocystis aeruginosa （exponential ≈ lag phase 〉 early stationary） rather than that of the bacterium itself. Our results also suggested the algicidal activity of strain A27 occurred via the production of extracellular algicidal compounds. Investigation of the algicidal compounds revealed that there were at least two different algicidal compounds produced by strain A27. These results indicated that strain A27 has great potential for use in the control of outbreaks of cyanobacterial blooms in Lake Taihu.

关 键 词：algicidal bacteria freshwater cyanobacteriat bloom Microcystis aeruginosa Exiguobacterium sp. A27 

分 类 号：X172[环境科学与工程—环境科学] TQ655[化学工程—精细化工]