固定化脂肪酶催化(R,S)-1-苯乙醇转酯化拆分反应及动力学研究  

Transesterification Resolution of (R,S)-1-phenylethanol over Immobilized Lipase and Its Kinetic Behavior

作　　者：张立根[1] 屈冠群[1] 彭洪根[1] 薛屏[1] 

机构地区：[1]宁夏大学天然气转化国家重点实验室培育基地,宁夏银川750021

出　　处：《宁夏大学学报（自然科学版）》2012年第3期265-269,共5页Journal of Ningxia University(Natural Science Edition)

基　　金："973"计划前期研究专项基金资助项目(2010CB534916);国家自然科学基金资助项目(21064005)

摘　　要：利用制备的含Fe3O4无机磁性复合物FSN固定假单胞菌脂肪酶(Pseudomonas sp Lipase,PSL),获得具有高活性、高对映体选择性和易分离的固定化酶PSL/FSN.乙酸乙烯酯做酰化试剂,PSL/FSN在正庚烷溶剂中催化(R,S)-1-苯乙醇转酯化拆分反应,40℃反应2h时,(S)-1-苯乙醇对映体过量值ees和(R)-乙酸-1-苯乙酯的对映体过量值eep均为99%,(R,S)-1-苯乙醇的转化率达到理论值。固定化酶PSL/FSN在70℃经2h热处理,其活性和对映选择性没有明显的下降;PSL/FSN重复使用10次,其活性和对映体选择性未出现衰减。基于实验数据,研究了固定化酶催化(R,S)-1-苯乙醇转酯化拆分反应的动力学行为,获得了(R,S)-1-苯乙醇转酯化拆分反应的动力学方程。The Pseudomonas sp Lipase （PSL） was immobilized on the inorganic magnetic compound support （FSN） containing nanoparticles Fe3O4. The immobilized lipase （PSL/FSN） has higher activity, conversion enantioselectivity and easier recovery advantages comparing with free lipase. PSL/FSN was used in the transesterification resolution of （R,S）-l-phenylethanol. The most suitable acyl donor, organic solvent and reaction temperature was vinyl acetate, respectively, refine n-heptane and 40 ℃. Under these conditions, the ee values of the substrate （S）-l-phenylethanol and the product （R）-1- phenylethyl acetate were both 99 % when reaction time is 2 h. After PSL/FSN was incubated 2 hours at 70 ℃, its activity and enantioselectivity was not significantly reduced. The catalytic performance of PSL/FSN was not decreased after PSL/FSN was reused for 10 times. Based on experiment data of transesterification resolution of （R, S）-1-phenylethanol over immobilized lipase reaction, we have investigated its kinetic behavior and got its kinetic equation.

关 键 词：固定化脂肪酶 (R S)-1-苯乙醇 转酯化反应 拆分 

分 类 号：TQ426.97[化学工程]