地塞米松诱导幼龄大鼠胸腺细胞凋亡模型  

EXPERIMENTAL STUDY ON DEXAMETHASONE-INDUCED APOPTOSIS OF INFANCY THYMOCYTES IN RATS

作　　者：王君[1] 仝小林[1] 迟永春[2] 王坤[2] 杨梦兰[1] 

机构地区：[1]中日友好医院中医科,北京100029 [2]中日友好医院临床医学研究所病理生理室,北京100029

出　　处：《中日友好医院学报》2000年第2期63-66,共4页Journal of China-Japan Friendship Hospital

基　　金：国家自然科学基金!3 9670 866

摘　　要：目的 :建立一个比较实用且成型时间短的地塞米松诱导大鼠胸腺细胞凋亡模型。方法 :给幼龄大鼠 ( 4周～ 5周 )腹腔注射地塞米松 ( 0 0 2 g/kg) ,采用形态学 (光镜和电镜 )、DNA琼脂糖凝胶电泳、流式细胞光度分析等方法研究 3、 6、 9、 1 5、 2 4h等不同注射时间胸腺细胞凋亡变化。结果 :在 1 5h内 ,地塞米松诱导的大鼠胸腺细胞凋亡百分率随时间延长 ,凋亡发生率从 6 2 %逐渐增至 58 5% ;注射地塞米松 1 5h ,在光镜和电镜下均观察到胸腺细胞凋亡的典型形态变化 ;DNA琼脂糖凝胶电泳呈现梯形条带 ;流式细胞光度分析可见凋亡峰出现。结论 :幼龄大鼠腹腔注射地塞米松 ( 0 0 2 g/kg) 1 5h可成功地诱导出一个比较理想的体内胸腺细胞凋亡模型。该模型成型时间短 ,适用于凋亡调控药物及其作用机理的研究。Objective:To develop an apoptotic model of thymocytes in infancy rats with an intraperitoneal injection of dexamethasone.Methods:Three,six,nine,15 and 24 hours after introperitoneal injection of dexamethasone(0 02 g/kg) in infancy rats,the thymocyte apoptosis was studied and analysed by using morphological method,DNA agarose gel electrophoresis and flow cytometry.Results:During 15 hours,the apoptotic rate of rat thymocytes induced by dexamethasone increased with time (6 2% 58 8%).The optimum time was 15 hours after injection for the inducing,in which typical apoptotic morphological changes,ladder in DNA agarose gel electrophoresis,and apoptotic peak in flow cytometric analysis were observed.Conclusion:The thymocyte apoptosis was successfully induced by introperitoneal injection of dexamethasone(0 02 g/kg),which provides a valuable reference for the mechanism study of thymocyte apoptosis and clinical practice.

关 键 词：大鼠 地塞米松 胸腺 凋亡 

分 类 号：R-332[医药卫生]