食管鳞状细胞癌组织中23种肿瘤相关蛋白的表达  

Expressions of 23 kinds of cancer-related protein in esophageal squamous cell carcinoma tissue

作　　者：崔文燕[1,2] 郭明[2] 原园[2] 周福有 张朋[1,2] 王伟鹏 赵学科[2] 陈元方 任景丽[6] 王立东[1,2] 

机构地区：[1]新乡医学院癌症研究中心,新乡453003 [2]郑州大学第一附属医院河南省食管癌重点开放实验室,郑州450052 [3]安阳肿瘤医院外科,安阳455000 [4]磁县医院病理科,磁县056500 [5]鹤壁市卫生局,鹤壁458030 [6]郑州大学第二附属医院病理科,郑州450014

出　　处：《郑州大学学报（医学版）》2012年第5期598-601,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30971133

摘　　要：目的:探讨食管鳞状细胞癌(食管鳞癌)组织中肿瘤相关蛋白表达与临床病理变化的关系。方法:采用免疫组化法检测679例食管鳞癌组织中细胞骨架结合蛋白Fascin、突触蛋白Syn和肿瘤抑制基因P63等23种肿瘤相关蛋白在癌组织中的表达,分析上述蛋白的表达与临床病理指标(淋巴结转移、分化程度及临床分期等)的关系。结果:23种蛋白中,食管癌组织中阳性表达率较高(≥80%)的蛋白是AE1/AE3、P63、CK5/6、CK、Ki67和MUC1,较低(≤30%)的是CD56、CTTN、Syn、S100、CgA和CK20。其中,Syn在淋巴结有转移组织比无转移组织表达升高(χ2=4.167,P=0.041),免疫蛋白CD56在低分化组织高于中高分化组织(P=0.017),细胞增殖抗原Ki67在中晚期组织高于早期(χ2=6.596,P=0.010);而与此相反,P63则在淋巴结无转移时表达上升(χ2=5.095,P=0.024),细胞黏附因子E-cad、Fascin、Ezrin、PLK1在中高分化组织中表达升高(P均<0.05),酪氨酸磷酸化蛋白CTTN和Vim在早期组织高于中晚期组织(P均<0.05)。结论:Syn、CD56和Ki67高表达可能与食管癌预后差有关;而P63、E-cad、Fascin、Ezrin、PLK1和Vim、CTTN高表达可能与食管癌预后好有关。Aim：To detect the expressions of 23 kinds of cancer-related protein and their relation with clinical pathological changes in 679 cases of esophageal squamous cell carcinoma（ESCC）,and to further understand the molecular basis for ESCC prognosis.Methods：Six hundred and seventy nine ESCC samples were surgically resected tissue.The expressions of 23 kinds of cancer-related protein,including Fascin, Syn,P63,et al were detected by immunohistochemical method.The relationship between the expressions of 23 kinds of protein and clinical pathological changes was analyzed.Results：Out of the 23 kinds of cancer-related protein,AE1/AE3, P63, CK5/6, CK, Ki67 and MUC1 （≥80%） had relatively higher positive expression rate in ESCC tissue,while CD56, CTTN, Syn, S100, CgA and CK20 （≤30%） had relatively lower positive expression rate.Syn expression was higher in metastasis tissue than in non-metastasis tissue（χ2=4.167,P=0.041）,CD56 expression was higher in poorly differentiated tissue than in high and moderate differentiated tissue （P=0.017）,and Ki67 expression was higher in advanced stage than in early stage （χ2=6.596,P=0.010）. In contrast,P63 expression was higher in ESCC tissue without metastasis （χ2=5.095,P=0.024）,the expressions of E-cad, Fascin, Ezrin,and PLK1 were higher in high and moderate differentiated tissue （P0.05）,and Vim and CTTN protein expression was higher in early stage than in advanced stage（P0.05）.Conclusion：The higher expressions of Syn, CD56 and Ki67 may be important candidates for ESCC with poor prognosis. However, the higher expressions of P63, E-cad, Fascin, Ezrin, PLK1, Vim, and CTTN may be important candidates for ESCC with better prognosis.

关 键 词：食管肿瘤 鳞状细胞癌 预后 肿瘤相关蛋白 淋巴结转移 分化程度 临床分期 

分 类 号：R735.1[医药卫生—肿瘤]