siRNA沉默Pim-3对T24细胞增殖和细胞周期的影响  

Effect of silencing Pim-3 with small interfering RNA on cell proliferation and cell cycle of T24 cells

作　　者：何文强[1] 尹继云[1] 孟晓[1] 王梅叶[1] 张文涛[1] 曲宪东[1] 李南南[1] 

机构地区：[1]武警河南总队医院泌尿外科,郑州450052

出　　处：《郑州大学学报（医学版）》2012年第5期681-684,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究小干扰RNA(siRNA)沉默Pim-3对膀胱癌细胞增殖和周期的影响。方法:膀胱癌T24细胞分为3组:未处理组(不作任何处理)、对照siRNA组(采用50nmol/L对照siRNA转染)和Pim-3siRNA组(采用50nmol/LPim-3siRNA转染)。采用Westernblot检测转染48hPim-3、CyclinD1、Cdk2、P21蛋白表达的变化,CCK-8试剂检测转染24、48、72、96h细胞的增殖速率,流式细胞术检测转染48h细胞周期的变化。结果:3组膀胱癌T24细胞Pim-3、CyclinD1、Cdk2、P21蛋白表达比较,差异有统计学意义(F=32.506、49.573、23.079、44.758,P<0.05),与未处理组和对照siRNA组相比,Pim-3siRNA组Pim-3、CyclinD1和Cdk-2蛋白的表达下降,P21蛋白表达升高(P<0.05)。转染Pim-3siRNA48、72和96h后,膀胱癌T24细胞的增殖速率均明显受到抑制(F=50.067、132.504、277.389,P<0.001)。3组G0/G1、S和G2/M期膀胱癌T24细胞比例比较,差异有统计学意义(F=107.970、20.039和66.872,P<0.05),Pim-3siRNA组G0/G1期膀胱癌T24细胞比例高于未处理组和对照siRNA组(P<0.05),而S和G2/M期膀胱癌T24细胞比例低于未处理组和对照siRNA组(P<0.05)。结论:Pim-3有望成为治疗膀胱癌潜在的分子靶点。Aim：To investigate the effect of silencing Pim-3 with small interfering RNA（siRNA）on cell proliferation and cell cycle of bladder carcinoma T24 cells.Methods：T24 cells were divided into 3 groups：untreated group,control siRNA group,and Pim-3 siRNA group.Pim-3 siRNA and control siRNA were utilized to transfect bladder carcinoma T24 cells by LipofectamineTM2000. Western blot was used to detect the protein expressions of Pim-3,Cyclin D1,Cdk2,and P21. CCK-8 kit and flow cytometry were used to examine cell proliferation and cell cycle, respectively.Results：The protein expressions of Pim-3,Cyclin D1,Cdk2,and P21 among the 3 groups had significant differences（F=32.506,49.573,23.079,44.758,P0.05）. The expressions of Pim-3,Cyclin D1,and Cdk2 decreased and P21 increased in Pim-3 siRNA group（P0.05）.The proliferation rate of T24 cells was obviously inhibited at 48,72,96 h after transfection with Pim-3 siRNA （F=50.067,132.504,277.389,P0.001）.The percentage of G0/G1,S,and G2/M phases cells among the 3 groups had significant differences（F=107.970,20.039,66.872,P0.05）.The percentage of G0/G1 phase of T24 cells in Pim-3 siRNA group was higher than those in untreated group and control siRNA group, whereas S and G2/M phases appeared the opposite result.Conclusion：Pim-3 may be a useful molecular target for therapy of bladder carcinoma.

关 键 词：PIM-3 膀胱癌 T24细胞 小干扰RNA 细胞增殖 细胞周期 

分 类 号：R730.21[医药卫生—肿瘤]