非小细胞肺癌患者外周血Th17细胞比例和血清IL-17水平的检测及其临床意义  

Detection and clinical significance of Thl7 cell proportion and expression of IL-17 in peripheral bloodof patients with non-small cell lung cancer

作　　者：赵树强[1] 贾春生[1] 王桂琴[2] 李淑勤[3] 

机构地区：[1]山西医科大学附属汾阳医院核医学科,032200 [2]山西医科大学微生物学与免疫学教研室 [3]山西医科大学汾阳学院临床系

出　　处：《肿瘤研究与临床》2012年第9期600-602,共3页Cancer Research and Clinic

基　　金：山西省吕梁市科技攻关项目（st2010143-1）

摘　　要：目的探讨非小细胞肺癌（NSCLC）患者外周血Th17细胞比例和白细胞介素．17（IL-17）水平的变化及其与患者临床病理的关系。方法收集60例原发未治疗的NSCLC患者（NSCLC组）和20例健康志愿者（对照组），采用流式细胞术（FCM）检测Th17细胞比例，采用酶联免疫吸附试验（ELISA）检测血清IL．17水平。结果NSCLC组Th17细胞占CD；T细胞比例高于对照组[（1．23±0．41）％比（1．05±0．28）％，t=1．679，P=0．097]，鳞状细胞癌患者外周血Th17细胞比例明显高于腺癌患者[（1．31±0．39）％比（1．09±0．41）％，t=2．093，P=0．041]。NSCLC组IL-17水平明显高于健康对照组[（21．11±7．87）pg／ml比（17．64±5．07）Pg／ml，t=2．280，P=0．027]，鳞状细胞癌患者血清IL-17表达水平明显高于腺癌患者f（23．114-8．73）Pg／ml比（18．54±6．38）Pg／ml，t=2．203，P=0．0321。Th17细胞比例、IL-17的表达水平随临床分期的进展呈下降趋势fF=3．151，P：0．032；F=4．132，P=0．010]。结论NSCLC患者外周血中Th17细胞比例和IL-17的表达与肿瘤病理分型、TNM分期相关，可能在NSCLG．的病程中发挥重要作用。Objective To investigate the relationship between the proportion of Thl7 cells and IL-17 expression in the peripheral blood and their clinical significance in patients with non-small cell lung cancer （NSCLC）. Methods The proportion of Th 17 cells determined by flow cytometry （FCM） and IL-17 detected by enzyme-linked immunosorbent assay （ELISA）. The data was analyzed from 60 untreated patients with NSCLC （NSCLC group） and 20 healthy volunteers （control group）. Results The Thl7 cells proportion in CD; T-cells in NSCLC group was significantly higher than that in control group [（1.23±0.41） % vs （1.05±0.28） %, t = 1.679, P = 0.097]. The expression levels of Thl7 cells in peripheral blood of patients with squamous cell carcinoma was significantly higher than that in adenoeareinoma patients [（1.31±0.39） % vs （1.09±0.41） %, t = 2.093, P = 0.041]. The concentration of IL-17 of patients with NSCLC was significantly higher than that in control group [（21.11±7.87） pg/m] vs （17.64±5.07） pg/ml, t = 2.280, P = 0.027]. The expression levels of serum IL-17 of squamous cell carcinoma patients was significantly higher than that of adenocarcinoma patients [（23.11± 8.73） pg/ml vs （18.54±6.38） pg/ml, t = 2.203, P = 0.032]. As clinical stage progressed, the proportion of Thl7 cells and IL-17 expression were significantly downtrend IF = 3.151, P = 0.032, F = 4.132, P = 0.010]. Conclusion The levels of Thl7 cells and 1L-17 in the peripheral blood of patients with NSCLC significantly correlated with the pathological types and TNM stage, might play an important role in the course of the disease.

关 键 词：b癌 非小细胞肺 流式细胞术 TH17细胞 白细胞介素-17 

分 类 号：R734.2[医药卫生—肿瘤]