气相色谱-串联质谱法测定牛奶中20种有机氯农药残留  

Determination of 20 Organochlorine Pesticides Residues in Milk by Gas Chromatography-Tandem Mass Spectrometry

作　　者：刘毅[1] 郑国灿[1] 王晶[1] 陈冬东[2] 朱美文[1] 彭光宇[1] 曹淑瑞[1] 王国民[1] 

机构地区：[1]重庆出入境检验检疫局重庆市进出口食品安全工程技术研究中心,重庆400020 [2]中国检验检疫科学研究院

出　　处：《检验检疫学刊》2012年第4期38-45,共8页Journal of Inspection and Quarantine

基　　金：质检公益性行业科研项目(201010073);重庆市科委科研项目(CSTC2009CB1012)

摘　　要：建立了牛奶中20种有机氯农药残留的气相色谱-串联质谱检测方法。样品采用正己烷-丙酮混合溶剂提取,凝胶渗透色谱和弗罗里硅土固相萃取联合净化,气相色谱-串联质谱测定。标准溶液在4μg/L-400μg/L范围内线性关系良好;方法的定量限为0.8μg/kg。20种有机氯农药在3个水平(0.8、2.0、10.0μg/kg)的加标回收率为62.2%-103.8%,相对标准偏差(RSDs)为3.4%-13.1%。该分析方法稳定可靠,灵敏度高,适合牛奶中多种有机氯农药残留量的检测。A gas chromatography-tandem mass spectrometry（GC-MS /MS） method was developed for the determination of 20 organochlorine pesticides residues in milk.The samples were extracted by mixed solvent of n-hexene and acetone,purified by gel permeation chromatography and magnesiumtrisilicate solid phase extraction column,then detected by GC-MS/MS.The calibration curves showed good linearity in the range of 4μg/L-400μg /L.The limits of detection were 0.8μg/kg.The recoveries of 20 organochlorine pesticides in milk at the three spiked levels of 0.8,2,10μg/kg were in the range of 62.2%-103.8% with the relative standard deviations（RSDs） of 3.4%-13.1%.The method is stable and reliable,high sensitivity.

关 键 词：气相色谱-串联质谱 牛奶 有机氯农药 残留量 

分 类 号：O657.63[理学—分析化学]