乳腺癌波形蛋白表达的病理学研究  

Pathological research on breast cancer with vimentin expression

作　　者：夏国庆 褚健 刘官成 李国民[2] 

机构地区：[1]南京军医学院病理学教研室,南京210099 [2]解放军第307医院病理科,北京100850

出　　处：《南京军医学院学报》2000年第2期77-79,共3页Journal of Nanjing Military Medical College

摘　　要：目的 :研究波形蛋白 (vimentin)在乳腺癌上皮中的表达及其与乳腺癌病理特征的关系。方法 :用免疫组化检测vimentin、增生细胞核抗原 (PCNA)、细胞角蛋白 (cytokeratin)等在乳腺癌上皮中的表达 ,用DNA斑点杂交检测c myc癌基因在乳腺癌组织中的表达 ,用葡聚糖包裹活性碳吸附法检测乳腺癌组织的雌激素受体 (ER)值。结果 :Vimentin可在 2 6 87%的乳腺癌组织中表达 ,其表达与乳腺癌的组织学分级、ER阴阳性和PCNA指数等一系列特征有关。Vimentin表达阳性的乳腺癌 ,其细胞形态与一般的乳腺癌上皮有所不同 ,细胞角蛋白阳性程度减弱 ,c myc癌基因较多发生扩增。结论 :Vimentin表达阳性的乳腺癌是一类恶性度较高、预后可能不良的乳腺癌。Objective:To investigate vimentin expression in breast cancer epithelia and its relationship with various pathological features of breast cancer. Methods:The immunohistochemistry was used to detect vimentin, proliferating cell nuclear antigen(PCNA) and cytokeratin expression in breast cancer epithelia. The DNA dot-blotting hybridization was used to detect the expressing status of c-myc oncogene amplification in breast cancer tissues, and the radio-labelling dextran-coated activated charcoal absorption was applied to assay the estrogen receptor (ER) quantity. Results:The vimentin could be expressed in 26 87% breast cancer tissues, and its expression correlated to histologic grade, ER status and PCNA index, etc. The breast cancer in which the vimentin expressed positively was found to be slightly different from the ordinary breast cancer epithelia in morphology, and the cytokeratin staining was also poor. The proto-oncogene c-myc amplified more frequently. Conclusion:The breast cancer with positive vimentin expression is a kind of breast cancer which is more malignant and probably with poor prognosis.

关 键 词：乳腺癌 波形蛋白 癌基因 病理 

分 类 号：R737.902[医药卫生—肿瘤]