真菌诱导白木香产生沉香的野外实验与分析  

Field Test and Analysis for Fungus Inducing the Formation of Agilawood from Aquilaria sinensis

作　　者：崔晋龙[1,2] 肖培根[1] 郭顺星[1] 

机构地区：[1]中国医学科学院-北京协和医学院药用植物研究所,北京100193 [2]山西大学应用化学研究所,太原030006

出　　处：《中国药学杂志》2012年第20期1614-1617,共4页Chinese Pharmaceutical Journal

基　　金：国际科技合作项目(2011DFA31260)

摘　　要：目的采用野外小规模实验和室内分析相结合的方法验证3株真菌YNAS04、YNAS06和YNAS08诱导白木香产生沉香的可行性。方法采用真菌诱导法诱导白木香产生沉香,利用气相-质谱联用技术(GC-MS)对诱导结香材料挥发油成分中的化合物进行检测,通过比对诱导材料高频菌与原接入菌的形态及核糖体基因内部转录间隔区序列(internal transcribed spacer,ITS)一致性来验证接入菌的有效性。结果 3株真菌定植于白木香6个月,YNAS04、YNAS06和YNAS08诱导产生材料的重量分别为(1.786±0.473),(2.964±0.492)和(3.615±0.591)g,高于空白对照(1.325±0.407)g和阴性对照(1.462±0.417)g。GC-MS检测到3株活性真菌诱导材料含有代表沉香品质的主要化学成分倍半萜类化合物和色原酮类似物。菌种验证结果表明,3株活性真菌在诱导材料中为优势菌,分别占分离菌种的90.0%,97.5%和91.25%。结论利用3株活性真菌诱导白木香产生沉香可以应用于实际生产,是可持续、高效生产沉香的良好方法。OBJECTIVE To investigate the feasibility of utilizing three fungi YNAS04,YNAS06 and YNAS08 to induce the formation of agilawood from Aquilaria sinensis in field and laboratory, respectively. METHODS Agarwood was obtained by inoculating the fungus in the stem of A. sinensis under natural conditions, and the main ingredients of the volatile oil from induced agilawood were analyzed by GC-MS. The authenticity of the used fungi was detected by the method of morphology and ribosomal internal transcribed spacer. RESULTS The weights of the induced materials from YNAS04, YNAS06 and YNAS08 were （ 1. 786 ± 0. 473 ）, （ 2. 964± 0. 492） and （3. 615±0. 591 ） g after six months, which were heavier than blank control （1. 325 ± 0. 407）g and negative control （ 1. 462 ±0.417） g, respectively. The fungus inoculated in the stem of A. sinensis and became the dominant fungi in the tissue under natural conditions. Sesquterpenoids and chromone analogues were detected from three fungi inducing materials of six months old. CONCLUSION Utilizing fungi to induce the formation of agilawood from Aquilaria sinensis has the advantages of spending less time and producing high-quality secondary metabolites. This method has important application foreground in large-scale agilawood produc-

关 键 词：白木香 沉香 真菌 诱导活性 气相色谱-质谱联用 ITS序列分析 

分 类 号：R284[医药卫生—中药学]