荷包猪一类缺失型SLA-3等位基因的克隆及生物信息学分析  

Cloning and Analyzing a Deleted Type of SLA-3 Allele in Hebao Pigs by Bioinformatics

作　　者：乔翠[1] 许崇波[1] 张兴会[2] 张文军[2] 郭丹[2] 张胜[2] 高凤山[1] 

机构地区：[1]大连大学生物工程学院生物化学和分子生物学实验室,大连116622 [2]辽宁省畜牧科学研究院,辽阳111000

出　　处：《生物技术通报》2012年第9期87-93,共7页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30972169);辽宁省博士启动基金(20081078);辽宁省教育厅科学计划项目(L2010018)

摘　　要：为研究荷包猪SLA-3基因特征,设计引物克隆了荷包猪SLA-3,经生物信息学软件分析其基因特征。结果显示,所克隆的荷包猪SLA-3基因(暂命名为SLA-3-HB02)碱基序列长度为1 417 bp,其中2-1 054为ORF区,共编码350个氨基酸,在其编码的氨基酸334-344处存在缺失。SLA-3-HB02与其他SLA-3、SLA-2及SLA-1等位基因群的氨基酸同源率分别为87.8%-98.6%、82.8%-87.8%和83.7%-90.0%。分子进化关系分析,SLA-3-HB02与美国的Hanford遗传距离较近;各功能区分析,SLA-3-HB02保留了HLA-A2等的部分功能基因位点,并与HLA-B15和H-2K1有一定的交叉识别位点。同源模建揭示SLA-3-HB02分子与HLA-A2及H-2K1 3D结构相似。To study the genetic characteristics of Hebao pigs, a pair of primers was designed to clone SLA-3 gene followed by analyzing its genetic characteristics by bioinformatics. The results were shown that the SLA-3 （ named as SLA-3-HB02 ）was 1 417 bp with an ORF domain which coded for 350 amino acids and there is a deletion between 334-344 amino acid sites. The amino acid identical ratios between SLA-3- HB02 and other SLA-3, SLA-2, and SLA-1 alleles were 87.8%-98.6%, 82.8%-87.8%, and 83.7%-90.0%, respectively. The phylogenetic tree of the SLA^3-HB02 demonstrated that it was close to Hanford breed of swine in USA. The SLA-3-HB03 preserved some functional sites of HLA-A2 and it also shared some functional sites to the HLA-B 15 and H-2Kd. By homology modeling, the 3-dimentional （ 3D ） structure of SLA- 3-HB02, HLA-A2 and H-2K1 was similar.

关 键 词：荷包猪 SLA-3基因 缺失型 等位基因 生物信息学 

分 类 号：S828[农业科学—畜牧学]