樱桃花变绿病植原体的分子鉴定  

Molecular identification of a phytoplasma associated with cherry virescence disease

作　　者：郑晓慧[1] 朱国翱 王连春[3] 朱建勋[3] 蔡红[3] 陈海如[3] 

机构地区：[1]西昌学院农业科学学院,西昌6150013 [2]四川省冕宁县植保植检站,西昌615600 [3]云南农业大学,云南省植物病理重点实验室,昆明650201

出　　处：《植物病理学报》2012年第5期546-550,共5页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(31060239)

摘　　要：植原体（phytoplasma）是一类没有细胞壁,不能人工培养,存在于植物筛管细胞中的类似植物病原细菌的原核生物。迄今为止,世界各地报道的1 000余种植物病害与植原体有关,引起的症状主要包括丛枝、黄化、花变绿、花变叶、花器退化等。Using nested PCR with phytoplasma universal primer pair for 16S rDNA and Elm yellows （EY） group-specific primer pair for ribosomal protein gene operon to detect phytoplasma associated with cherry virescence （ChV） disease in Mianning County, Sichuan Province. DNA fragments about 1.2 kb were amplified from the total DNA of diseased cherry samples, respectively. After cloning, necleotide sequencing and searching similar sequences of BLAST in GenBank, construction of phylogenetic tree and online analysis of iPhyClassifier for 16S rDNA were made. The results indicated that the phytoplasma ChV was related to ＇ Candidatus Phytoplasma ziziphi＇ and belonged as variant of subgroup 16SrV-B. The comparative sequece and phylogenetic analyses of ribosomal protein gene revealed that the ChV phytoplasma represented a new rp gene lineage, designated as rpV-N. Phylogenetic analyses of rp gene sequences allowed clearer distinctions between ChV and closely related strains than did analysis of 16S rDNA.

关 键 词：植原体 分子鉴定 变绿 植物病原细菌 桃花 人工培养 原核生物 植物病害 

分 类 号：S436.65[农业科学—农业昆虫与害虫防治]