栓线长度、直径及大鼠体重与栓线法大鼠局灶性脑缺血模型关系的研究  

The relation of rat MCAO model quality to thread diameter,length and body weight of the rats

作　　者：辛世萌[1] 刘远洪[2] 聂志余[1] 

机构地区：[1]大连医科大学第二临床学院神经内科,辽宁大连116027 [2]河南省濮阳市人民医院神经内科,河南濮阳457000

出　　处：《大连医科大学学报》2000年第2期105-107,共3页Journal of Dalian Medical University

摘　　要：目的 :为提高栓线法大鼠局灶性脑缺血 (MCAO)模型的成功率和稳定性 ,探讨大鼠体重、栓线插入长度和直径与MCAO模型成功的关系。方法 :利用 2× 2× 2析因设计 ,通过检测神经功能缺陷评分和脑梗塞体积 ,分析大鼠体重、栓线插入长度和直径与MCAO模型成功的关系。结果 :大鼠体重、栓线直径两水平之间有差异 (P <0 0 5) ,栓线插入长度两水平之间无差异(P <0 0 5) ;大鼠体重、栓线直径之间有正交互作用 (P <0 0 5) ,其余两两之间以及三者之间无交互作用 (P >0 0 5)。结论 :最佳造模方案是大鼠体重 2 50～ 30 0g、线长 17mm或 18mm、线直径 0 2 8mm ,该方案制作的MCAO模型成功率高 ,重复性好。Objective:To clarify the relation of middle cerebral artery occlusion [MCAO(intraluminal thread method)]model to the filament diameter and length and rat weight.Methods:The relation of the filament diameter and length,rat weight was analyzed with 2×2×2 factorial design,via assessing neurlolgical deficit scores.Results:The neurological deficit scores betwwen the two levels of the rat weight or the fliament diameter were significantly different (P<0 05).No significant difference was found between the two levels of the filament length(P>0 05). A positive interaction was shown between the rat weight and the filament diameter(P<0 05),but no interaction among other factors.Conclu-sion:The reliability and reproducibilty of this MCAO model were greatly improved,when following the optimal method(rat weight 250g-300g,filament diameter 0 28 mm and filament length 17 mm or 18 mm. [WT5HZ]

关 键 词：栓线插入长度 栓线直径 局灶性脑缺血 动物模型 

分 类 号：R743[医药卫生—神经病学与精神病学]