HBV-DNA在肝细胞癌血清和肝组织中的差异检测  

Detection of HBV-DNA in liver tissue and serum of HCC patient

作　　者：张胜亮[1] 魏巍[1] 张耀铮[1] 李士军[2] 高海德[3] 袁宏[2] 

机构地区：[1]大连医科大学临床生化教研室,辽宁大连116027 [2]大连医科大学第一临床学院检验科,辽宁大连116011 [3]大连医科大学第四临床学院外科,辽宁大连116001

出　　处：《大连医科大学学报》2000年第2期133-135,共3页Journal of Dalian Medical University

摘　　要：利用PCR及ELISA方法联合检测HBV在肝细胞癌肝组织中及血清标本中的感染情况。HBV -DNA在肝组织与血清标本中的检出率不同 ,分别为 81 3%和 37 5%。血清HBV -DNA的检出与HBsAg不一致。提示血清和肝组织中的HBV -DNA的差异检测结合ELISA可以初步显示HBV在体内的感染情况。HBV was detected in HCC patients' serum and liver tissue by using PCR and ELISA.The rate of HBV-DNA detected in liver tissue and serum were 81 3% and 37 5% respectively.The different results were determined between HBV-DNA and HBsAg in serum.These results suggested that the different types of HBV infection in HCC were determined more accurately by combining detection of HBV-DNA in different tissues and anti-HBV in serum. [WT5HZ]

关 键 词：乙型肝炎病毒 肝肿瘤 HBV-DNA 血清 检测 

分 类 号：R735.7[医药卫生—肿瘤] R446.112[医药卫生—临床医学]