4-苯乙烯磺酸马来酸酐共聚物抑制HSV-2感染研究  

Antiviral effect of PSM against herpes simplex virus type 2

作　　者：陈钰[1] 宋思维[1] 邱敏[1] 吴稚伟[1] 

机构地区：[1]南京大学医学院公共健康医学中心,江苏南京210093

出　　处：《东南大学学报（医学版）》2012年第5期563-566,共4页Journal of Southeast University(Medical Science Edition)

基　　金：科技部国际合作项目(2009DFA31260);十二五传染病重大专项项目(2012ZX10001007-009);上海市科学技术委员会(纳米专项)项目(1052nm05600)

摘　　要：目的:探索4-苯乙烯磺酸马来酸酐共聚物(PSM)对单纯疱疹病毒Ⅱ型(HSV-2)感染细胞或小鼠的抑制作用。方法:建立Vero-ICP10细胞系,通过Luciferase检测不同浓度PSM对感染细胞中HSV-2的抑制作用,并通过In cell western技术检测HSV-2 gD蛋白表达,确定不同PSM浓度对HSV-2 gD蛋白表达的抑制作用;建立HSV-2阴道感染小鼠模型,观察PSM对HSV-2经生殖道感染小鼠的抑制作用。结果:Luciferase实验和In cell western实验结果均表明PSM具有抗HSV-2感染细胞的作用;小鼠模型中,5%的PSM能有效防止小鼠感染HSV-2(P<0.001)。结论:PSM在体内和体外模型中均可以抑制HSV-2感染。Objective： To investigate the antiviral effect of polymer of styrenesulfonic acid and maleic acid（4-styrenesulfonic acid-co-maleic acid）（PSM） against herpes simplex virus type 2（HSV-2）.Methods： A Vero-ICP10 cell line containing virus-controlled Luciferase as indicator was established for analyzing the effect of different concentrations of PSM on HSV-2 infection.An In cell western assay was also used for detecting intracellular viral protein expression.PSM inhibitory activity was further evaluated in a HSV-2 vaginal challenge murine model.Results： The results of in vitro analyses showed that PSM could protect cell from HSV-2 infection,and the murine model showed that 5% PSM protected 95% of the animals from HSV-2 infection（P〈0.001）.Conclusion： PSM is a potential agent for the prevention of HSV-2 infection through sexual route.

关 键 词：4-苯乙烯磺酸马来酸酐共聚物 单纯疱疹病毒2型 HSV-2阴道感染小鼠模型 抗病毒 

分 类 号：R-33[医药卫生]