α-细辛醚治疗前后海人酸致癎大鼠海马差异表达蛋白质的筛选研究  

Effects of α-asarone on expressions of proteins in hippocampus of rats with seizures induced by kainic acid

作　　者：谭明会[1] 吴原[1] 刘秋弟[1] 叶洁梅[1] 黄金山[1] 韦兴[1] 李偲俊[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,南宁530021

出　　处：《中国现代神经疾病杂志》2012年第5期589-593,共5页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：国家自然科学基金资助项目(项目编号:81160167)~~

摘　　要：目的应用蛋白质芯片技术筛选海人酸致癎大鼠于α-细辛醚治疗前后海马区差异表达蛋白质,寻找α-细辛醚治疗癫癎的药物作用靶点。方法健康成年SD大鼠20只,随机分为模型组和α-细辛醚组,经腹腔注射海人酸制备癫癎模型,选择Racine发作标准达Ⅳ～Ⅴ级的大鼠作为观察动物,BCA法分别测量o-细辛醚治疗前后海马组织差异表达的蛋白质水平,以INR≥1.30表示蛋白质表达水平上调、INR≤0.77表示蛋白质表达水平下调。结果共发现35种差异表达的蛋白质,与模型组大鼠相比,α-细辛醚组大鼠海马组织共有5种高表达蛋白质、30种低表达蛋白质,其中大多数蛋白质参与了机体的细胞信号转导、细胞周期调控、基因转录及调控、细胞凋亡和神经生物学等重要生物功能。结论α-细辛醚可调节由海人酸诱发的SD大鼠海马组织中多种蛋白质的表达变化,提示α-细辛醚具有多个药物治疗靶点。Objective To explore the effects of α-asarone on expressions of proteins in hippoeampus of rats with seizures induced by kainie acid （KA）, and find the drug action targets of α-asarone. Methods Twenty healthy male Sprague-Dawley （SD） rats were given KA by intraperitoneal injection to induce epilepsy, and were randomly divided into 2 groups： the KA group （n = 10） and α-asarone （24 mg/kg） group （n -- 10）. In α-asarone group α-asarone were administered once daily by intraperitoneal injection in 7 consecutive days. Bicinchoninic acid （BCA） method was used to measure the protein expression in hippocampus before and after α-asarone therapy. The endogenous international normalized ratio （INR） was calculated according to the standardization of hybridization fluorescence signal strength, and was used to measure the difference of protein expression between the two groups. INR I〉 1.30 indicated the protein expression up- regulated while INR ≤ 0.77 indicated the protein expression down- regulated. Results Thirty-five differentially expressed proteins were found. In comparison with KA group 5 proteins presented higher expression, while 30 lower expression in α-asarone group, and most of them involved in signal transduction, cell cycle regulation, gene transcription and regulation, apoptosis, and neurobiological functions. Conclusion α-Asarone may regulate a variety of protein expression in hippoeampus of rats with seizures induced by kainie acid, and it may indicate that the drug has multiple therapeutic targets.

关 键 词：石菖蒲 细辛醚 癫癎 寡核苷酸探针 蛋白质类 疾病模型 动物 

分 类 号：R742.1[医药卫生—神经病学与精神病学]