Genistein与5-FU联合对人肝癌细胞MHCC97-L的抗增殖作用  

Genistein combined with 5-FU inhibits cell proliferation in human hepatocellular cancer cell line MHCC97-L

作　　者：刘丹[1] 赵忠新 

机构地区：[1]齐齐哈尔医学院生物遗传教研室,黑龙江省齐齐哈尔市161006 [2]齐齐哈尔市建华医院普外科,黑龙江省齐齐哈尔市161006

出　　处：《世界华人消化杂志》2012年第26期2474-2478,共5页World Chinese Journal of Digestology

基　　金：黑龙江省卫生厅科研课题基金资助项目;No.2010-226~~

摘　　要：目的:探讨Genistein与5-FU联合对人肝癌MHCC97-L细胞凋亡的诱导作用.方法:采用MTT法、倒置显微镜、Hoechst 33342荧光染色技术研究Genistein、5-FU、Genistein与5-FU联合3组药物不同浓度作用于体外培养的人肝癌MHCC97-L细胞后生长抑制及诱导凋亡的形态学变化.结果:Genistein、5-FU单用及联用可抑制肝癌MHCC97-L细胞的增殖,其抑制率与药物剂量和作用时间呈依赖关系;48h单用时的IC50(Genistein)为174.17mol/L,IC50(5-FU)为40.02mol/L;48h联用时的IC50(Genistein)为66.03mol/L、IC50(5-FU)为16.51mol/L;倒置显微镜结果显示,药物组细胞密度均明显下降,贴壁细胞出现皱缩、变圆、胞浆混浊及"空泡"现象,尤以联用药物组变化最为明显;荧光染色结果显示,药物组部分细胞核呈现致密亮蓝色荧光的为凋亡细胞,凋亡指数Genistein组为17.55%,5-FU组为15.63%,联用组为30.38%.结论:两种药物单独使用均可对人肝癌MHCC97-L细胞的生长具有明显抑制作用,联合用药时,Genistein可以增强5-FU的疗效,诱导细胞凋亡可能是其作用机制之一.AIM：To explore whether genistein and 5-FU has synergistic inhibitory effect on the proliferation of human hepatic cancer cells （MHCC97-L）. METHODS：MTT method was used to assay the biological activities of different concentrations of genistein and 5-FU in MHCC97-L cells. The inverted microscope was used to observe the influence of combined genistein and 5-FU on the morphological changes of MHCC97-L cells. After the cells were stained with Hoechst 33342 and observed under a fluorescence microscope, apoptosis index was calculated. RESULTS：Both genistein and 5-FU could effectively inhibit the proliferation of MHCC97-L cells in a dose and time-dependent manner. When used alone, the IC50 （48 h） of genistein and 5-FU for MHCC97-L cells was 174.17 mol/L and 40.02 mol/L, respectively. When used in combination, the IC50 of genistein and 5-FU was 66.03 mol/L and 16.51 mol/L. The cell density in the combination group was lower than the two monotherapy groups. Morphologic characteristics of apoptotic cells, such as cytoplasmic clouding, cell shrinkage and cytoplasmic vacuolation, were observed. Typical apoptosis was confirmed by fluorescence microscopy. The apoptosis index was 17.55% in the genistein group, 15.63 in the 5-FU group, and 30.38% in the combination group. CONCLUSION：Genistein and 5-FU can exert synergistic inhibitory effects on the growth of MHCC97-L cells possibly via mechanism associated with inducing apoptosis.

关 键 词：染料木黄酮 氟尿嘧啶 MHCC97-L 细胞凋亡 

分 类 号：R735.7[医药卫生—肿瘤]